Amphotericin B induced structural changes of the erythrocyte membrane.

Incubation of human erythrocytes in isotonic NaCl-sucrose medium with a relative high concentration of amphotericin B results in the occurrence of distinct structures on the membrane faces as visible by freeze-fracturing. Such kind of structures is known to be formed by phase separation of the lipid components (domain formation). The induction of phase separation is considered to be an additional effect of amphotericin B.