Glaser R
J Membr Biol. 1982;66(2):79-85. doi: 10.1007/BF01868484.
In isotonic 30 mM NaCl-saccharose solution, human red blood cells with intact membrane and normal inside ionic content (C-state) indicate a transmembrane potential between +30 mV (at pH 7.4) and +46 mV (at pH 5.1). After treatment with amphotericin B or nystatin as ionophores, a Donnan equilibrium (D-state) will be reached with the same potential at pH 5.1 but a sharp drop down to -20 mV will occur at pH 7.4. Concerning the erythrocyte shape at these states, a stomatocyte-echinocyte transformation takes place, in correlation with the potential shift. Stomatocytes formed at delta psi greater than +25 mV, echinocytes at delta psi less than +25 mV. At potentials lower than +5 mV, no further effect can be observed. This process is reversible. Neuraminidase treatment as well as outside EDTA do not influence this process significantly. Human serum albumin in concentrations of 2% stabilizes the stomatocytes.
在等渗的30 mM氯化钠 - 蔗糖溶液中，具有完整细胞膜和正常内部离子含量的人红细胞（C态）显示出跨膜电位在 +30 mV（pH 7.4时）和 +46 mV（pH 5.1时）之间。在用两性霉素B或制霉菌素作为离子载体处理后，将达到唐南平衡（D态），在pH 5.1时具有相同电位，但在pH 7.4时会急剧下降至 -20 mV。关于这些状态下的红细胞形状，会发生口形细胞 - 棘状细胞转化，与电位变化相关。当膜电位差大于 +25 mV时形成口形细胞，膜电位差小于 +25 mV时形成棘状细胞。在电位低于 +5 mV时，观察不到进一步的影响。这个过程是可逆的。神经氨酸酶处理以及外部添加乙二胺四乙酸（EDTA）对该过程没有显著影响。浓度为2%的人血清白蛋白可使口形细胞稳定。
