Zhang Siqi, Gao Yuan, Wang Danyang, Chi Yongjie, Wu Fang, Wang Lianyan, Jin Hailan
Key Laboratory of Bio-Based Material Science & Technology, Ministry of Education, Northeast Forestry University, Harbin 150040, China.
Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.
J Funct Biomater. 2026 Jan 30;17(2):71. doi: 10.3390/jfb17020071.
Skin aging could lead to dermal collagen loss and elastic fiber degradation, ultimately manifesting as skin laxity. We aimed to counteract this by using poly-L-lactic acid (PLLA) microsphere (MS)-based fillers to facilitate long-term volume restoration through collagen regeneration. However, conventional MSs exhibit limitations such as broad size distribution and surface irregularities, which are frequently associated with significant adverse reactions. This study employed shirasu porous glass (SPG) membrane emulsification to fabricate uniform and well-shaped polyethylene glycol-block-poly (L-lactic acid) (PEG-PLLA) MSs. A single-factor experiment was employed to optimize the parameters. The optimal preparation conditions for PEG-PLLA MSs were as follows: PEG-PLLA concentration of 40 mg/mL, polyvinyl alcohol (PVA) concentration of 0.5%, and magnetic stirring speed of 200 rpm. Under the optimal conditions, the average particle size of PEG-PLLA MSs was 58.982 μm, and the span value (SPAN) was 1.367. In addition, a cytotoxicity assay was performed, and the results revealed no significant toxicity of the MSs toward L929 mouse fibroblasts at concentrations below 500 μg/mL. Furthermore, PEG-PLLA MSs significantly enhanced the production of key extracellular matrix (ECM) components-type I collagen (Col-I), type III collagen (Col-III), and hyaluronic acid (HA)-while simultaneously alleviating cellular oxidative stress responses. This work offers a reliable and reproducible fabrication strategy for developing biocompatible MS fillers with controllable particle sizes.
皮肤老化会导致真皮层胶原蛋白流失和弹性纤维降解，最终表现为皮肤松弛。我们旨在通过使用基于聚-L-乳酸（PLLA）微球（MS）的填充剂来对抗这种情况，以促进胶原蛋白再生实现长期的体积恢复。然而，传统的微球存在诸如尺寸分布宽泛和表面不规则等局限性，这些常常与显著的不良反应相关。本研究采用白土多孔玻璃（SPG）膜乳化法制备均匀且形状良好的聚乙二醇-聚（L-乳酸）（PEG-PLLA）微球。采用单因素实验对参数进行优化。PEG-PLLA微球的最佳制备条件如下：PEG-PLLA浓度为40mg/mL，聚乙烯醇（PVA）浓度为0.5%，磁力搅拌速度为200rpm。在最佳条件下，PEG-PLLA微球的平均粒径为58.982μm，跨度值（SPAN）为1.367。此外，进行了细胞毒性试验，结果表明在浓度低于500μg/mL时，微球对L929小鼠成纤维细胞无明显毒性。此外，PEG-PLLA微球显著增强了关键细胞外基质（ECM）成分——I型胶原蛋白（Col-I）、III型胶原蛋白（Col-III）和透明质酸（HA）的产生，同时减轻了细胞氧化应激反应。这项工作为开发具有可控粒径的生物相容性微球填充剂提供了一种可靠且可重复的制造策略。
