用放射性同位素稀释法测定某些糖蛋白中的氨基葡萄糖和半乳糖胺。
The determination of glucosamine and galactosamine in some glycoproteins by radioisotope dilution.
作者信息
Graham E R, Neuberger A
出版信息
Biochem J. 1968 Oct;109(4):645-50. doi: 10.1042/bj1090645.
Abstract
- The principle of radioisotope dilution was applied on a semi-micro scale to the determination of glucosamine and galactosamine in some glycoproteins, such as immunoglobulins, a urinary glycoprotein and blood-group-specific substances. 2. The glycoprotein was hydrolysed in the presence of [1-(14)C]glucosamine or [1-(14)C]galactosamine or both. The amino sugars were made to react with naphthyl isothiocyanate and the products formed were isolated by the method of Scott (1962). The specific radioactivities determined from liquid-scintillation counting and the extinction at 240mmu or 222mmu were used to calculate the content of amino sugars in the protein analysed. 3. Where the values could be compared with those found by other workers, differences were in general not very great. The advantages of the method are that high concentrations of acid can be employed and undesirable side reactions, which may occur with the free sugars, do not affect the results. A potential source of error of the method is discussed.
摘要
- 放射性同位素稀释原理在半微量规模上应用于某些糖蛋白中氨基葡萄糖和半乳糖胺的测定，这些糖蛋白如免疫球蛋白、一种尿糖蛋白和血型特异性物质。2. 糖蛋白在[1-(14)C]氨基葡萄糖或[1-(14)C]半乳糖胺或两者存在的情况下进行水解。使氨基糖与异硫氰酸萘酯反应，并通过斯科特（1962年）的方法分离形成的产物。由液体闪烁计数测定的比放射性以及在240毫微米或222毫微米处的吸光度用于计算所分析蛋白质中氨基糖的含量。3. 在可以与其他研究者得到的值进行比较的情况下，一般差异不是很大。该方法的优点是可以使用高浓度的酸，并且可能与游离糖发生的不良副反应不会影响结果。讨论了该方法潜在的误差来源。
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J Exp Med. 1952 Jan;95(1):71-97. doi: 10.1084/jem.95.1.71.
3
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