Lev R, Loud A V, Laitio M, Orlic D
Int J Gynaecol Obstet. 1979 Sep-Oct;17(2):172-8. doi: 10.1002/j.1879-3479.1979.tb00143.x.
When injected into the amniotic sacs of fetal rats in late pregnancy, 3H-leucine is rapidly absorbed and incorporated into fetal proteins as determined by liquid scintillation spectrometry. Peak values in the fetal liver and pancreas occur around four hours after injection. Approximately 21% of the radioisotope is retained in the injected fetus at 24 hours. The remainder escapes into the maternal circulation and accounts for a gradual increase in levels in noninjected fetuses from the same litter and for persistently high counts found in maternal serum as late as 24 hours after the injection. Specific activity of the radioisotope in fetal tissues is considerably higher (100- to 200-fold) when 3H-leucine is injected inth the amniotic sac rather than intravenously into the mother. The possible treatment of various fetal disorders by means of intra-amniotic injections is discussed.
在妊娠后期将³H - 亮氨酸注入胎鼠羊膜囊时，通过液体闪烁光谱法测定，它会迅速被吸收并掺入胎儿蛋白质中。注入后约四小时，胎儿肝脏和胰腺中的³H - 亮氨酸达到峰值。24小时时，注入的胎儿中约21%的放射性同位素被保留。其余部分进入母体循环，导致同一窝未注入胎儿体内的³H - 亮氨酸水平逐渐升高，并且在注射后24小时母体血清中仍持续存在高计数。当³H - 亮氨酸通过羊膜囊注射而非静脉注射给母体时，胎儿组织中放射性同位素的比活性要高得多（100至200倍）。文中还讨论了通过羊膜腔内注射治疗各种胎儿疾病的可能性。
