迟发型超敏反应中可溶性抗原和淋巴细胞介导的细胞毒性。I. 该现象的特征
Cytotoxicity mediated by soluble antigen and lymphocytes in delayed hypersensitivity. I. Characterization of the phenomenon.
作者信息
Ruddle N H, Waksman B H
出版信息
J Exp Med. 1968 Dec 1;128(6):1237-54. doi: 10.1084/jem.128.6.1237.
Abstract
In the presence of specific antigen, lymph node cells from inbred rats with delayed hypersensitivity to tuberculoprotein, bovine gammaglobulin, and egg albumin produced progressive destruction of monolayers of rat embryo fibroblasts in tissue culture, first apparent at 48 hr and maximal at 72 hr. The effect was specific and did not depend on a genetic difference between the lymph node cells and target cells. It required antigen concentrations equal to or greater than 1.25 microg/ml and lymphocyte: target cell ratios of approximately 10 or 20:1. It could be evaluated both by a plaquing technique and by cell enumeration with an electronic particle counter.
摘要
在存在特异性抗原的情况下，对结核蛋白、牛γ球蛋白和卵清蛋白有迟发型超敏反应的近交系大鼠的淋巴结细胞，在组织培养中会对大鼠胚胎成纤维细胞单层产生进行性破坏，这种破坏在48小时时首次显现，72小时时达到最大程度。这种效应具有特异性，并不取决于淋巴结细胞与靶细胞之间的遗传差异。它需要抗原浓度等于或大于1.25微克/毫升，淋巴细胞与靶细胞的比例约为10或20:1。它既可以通过噬斑技术进行评估，也可以通过电子粒子计数器进行细胞计数来评估。
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J Exp Med. 1936 Aug 31;64(3):339-53. doi: 10.1084/jem.64.3.339.
2
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J Exp Med. 1965 Jul 1;122(1):143-66. doi: 10.1084/jem.122.1.143.
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