Okazaki M, Moo-Young M
Biotechnol Bioeng. 1978 May;20(5):637-63. doi: 10.1002/bit.260200503.
A generalized mechanistic model for the enzymatic hydrolysis of cellulose is developed and expressed mathematically. The model is based on Michaelis--Menten-type kinetics for concurrent random and endwise attack of the substrate involving end-product inhibitions and three types of enzymes: an endo-beta-1,4-glucanase, an exo-beta-1,4-glucanase, and beta-glucosidase. Basic parameters of the model which can explain synergistic and other effects observed experimentally are quantified and discussed. It is shown that cellulose degradation kinetics are expected to be strongly affected by the ratio of endo- to exocellulases in the reaction mixture as indicated by previous experimental data, and the substrate degree of polymerization, a factor not fully appreciated in previous studies, which appear to be overridingly important in many practical cases.
建立了纤维素酶促水解的广义机理模型，并进行了数学表达。该模型基于米氏动力学，用于底物的同时随机和端向攻击，涉及终产物抑制以及三种类型的酶：内切β-1,4-葡聚糖酶、外切β-1,4-葡聚糖酶和β-葡萄糖苷酶。对模型的基本参数进行了量化和讨论，这些参数可以解释实验中观察到的协同效应和其他效应。结果表明，如先前实验数据所示，纤维素降解动力学预计会受到反应混合物中内切酶与外切酶比例的强烈影响，而底物聚合度是先前研究中未充分认识到的一个因素，在许多实际情况中似乎至关重要。
