Ongun A, Thomson W W, Mudd J B
J Lipid Res. 1968 Jul;9(4):416-24.
Reaction of osmium tetroxide with isolated spinach chloroplasts fixed completely the glycolipids, phosphatidyl glycerol, and phosphatidyl choline. Under the same reaction conditions only 30% of the chlorophyll was fixed. Reaction of potassium permanganate with isolated spinach chloroplasts fixed more than 90% of the glycolipids, phosphatidyl glycerol, and phosphatidyl choline, provided the reaction period was long enough. Potassium permanganate also fixed the chlorophyll. Reaction of osmium tetroxide and potassium permanganate with isolated (14)C-lipids from Chlorella pyrenoidosa fixed 59% and 66% of the radioactivity, respectively. The lipids that were not fixed included sterols and pigments. Electron micrographs show that chloroplasts extracted with chloroform-methanol after fixation in osmium tetroxide or potassium permanganate differ from those dehydrated with acetone mainly in that in the former, osmiophilic globules have been removed and there seems to be some fusion of the boundary membranes and grana membranes. These effects may be due to the extraction of unfixed, neutral lipids such as sterols and quinones.
四氧化锇与分离出的菠菜叶绿体反应，能使糖脂、磷脂酰甘油和磷脂酰胆碱完全固定。在相同反应条件下，只有30%的叶绿素被固定。如果反应时间足够长，高锰酸钾与分离出的菠菜叶绿体反应能固定超过90%的糖脂、磷脂酰甘油和磷脂酰胆碱。高锰酸钾也能固定叶绿素。四氧化锇和高锰酸钾与来自小球藻的分离出的（14）C - 脂质反应，分别固定了59%和66%的放射性。未被固定的脂质包括固醇和色素。电子显微镜照片显示，用四氧化锇或高锰酸钾固定后再用氯仿 - 甲醇提取的叶绿体，与用丙酮脱水的叶绿体不同，主要在于前者嗜锇小球已被去除，且边界膜和基粒膜似乎有一些融合。这些效应可能是由于未被固定的中性脂质如固醇和醌类被提取所致。
