用于电子显微镜观察的大鼠肝脏线粒体分离制备
Preparation of isolated rat liver mitochondria for electron microscopy.
作者信息
Butler W H, Judah J D
出版信息
J Cell Biol. 1970 Feb;44(2):278-89. doi: 10.1083/jcb.44.2.278.
Abstract
A comparative study of the fixation of isolated rat liver mitochondria was undertaken. If the criterion is adopted that after processing, the mitochondria should resemble as closely as possible rat liver mitochondria in situ, the procedure found to produce such preservation was that of fixation in suspension in veronal-buffered 2% potassium permanganate. Fixation in osmium tetroxide produced variable results, while mitochondria fixed in glutaraldehyde were contracted. We suggest that in cases where fixation procedures modify the morphological appearance of mitochondria, the significance of such changes must be treated with caution.
摘要
对分离的大鼠肝脏线粒体的固定进行了一项比较研究。如果采用这样的标准，即经过处理后，线粒体应尽可能与原位的大鼠肝脏线粒体相似，那么发现能产生这种保存效果的程序是将其悬浮在巴比妥缓冲的2%高锰酸钾中进行固定。用四氧化锇固定产生的结果不一，而用戊二醛固定的线粒体则会收缩。我们建议，在固定程序改变线粒体形态外观的情况下，必须谨慎对待这种变化的意义。
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