Day D F, Marceau-Day M L, Ingram J M
Can J Microbiol. 1978 Feb;24(2):196-9. doi: 10.1139/m78-035.
Lysozyme (EC 3.2.1.17) complexes with extracted Pseudomonas aeruginosa LPS in two distinct stages. The initial stage does not produce turbidity detectable by nephelometry (measured as nephelos units (N) per time) but does permit low-speed sedimentation of the lysozyme-lipopolysaccharide (LPS) complex. This association is 100% disrupted by the action of 0.1 M Mg2+. Monovalent cations at equal ionic strength to the Mg2+ concentration used for these studies failed to alter significantly the lysozyme-LPS complex, indicating that the role of Mg2+ was not strictly an ionic one. The study of lysozyme-LPS complexes may provide a model system for investigating in vivo protein-LPS interactions.
溶菌酶（EC 3.2.1.17）与提取的铜绿假单胞菌脂多糖（LPS）结合分两个不同阶段。初始阶段不会产生比浊法可检测到的浊度（以每次的浊度单位（N）衡量），但允许溶菌酶 - 脂多糖（LPS）复合物进行低速沉降。这种结合在0.1 M Mg2 + 的作用下100%被破坏。在这些研究中，与所使用的Mg2 + 浓度具有相同离子强度的单价阳离子未能显著改变溶菌酶 - LPS复合物，这表明Mg2 + 的作用并非严格的离子作用。对溶菌酶 - LPS复合物的研究可能为研究体内蛋白质 - LPS相互作用提供一个模型系统。
