Jarrell K, Kropinski A M
J Virol. 1977 Sep;23(3):461-6. doi: 10.1128/JVI.23.3.461-466.1977.
Bacteriophage E79 was shown to interact with the lipopolysaccharide (LPS) of Pseudomonas aeruginosa strain PAO. LPS isolated from an E79-sensitive, smooth strain inactivated the phage, exhibiting a Phl50 value (concentration of LPS that caused a 50% decrease in the titer of phage during 1 h of incubation at 37 degrees C) of 0.04 microgram/ml, whereas the LPS isolated from a rough mutant derived from the wild type showed no neutralizing activity towards E79. EDTA and sodium deoxycholate were demonstrated to abolish the neutralizing capacity of the smooth LPS. One E79 receptor site was shown to be equivalent to 10(-16) g of LPS.
噬菌体E79被证明可与铜绿假单胞菌PAO菌株的脂多糖(LPS)相互作用。从对E79敏感的光滑菌株中分离出的LPS可使噬菌体失活,其Phl50值(在37℃孵育1小时期间导致噬菌体滴度降低50%的LPS浓度)为0.04微克/毫升,而从野生型衍生的粗糙突变体中分离出的LPS对E79没有中和活性。已证明EDTA和脱氧胆酸钠可消除光滑LPS的中和能力。一个E79受体位点相当于10^(-16)克LPS。