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铜绿假单胞菌PAO菌株中噬菌体E79细胞壁受体的鉴定

Identification of the cell wall receptor for bacteriophage E79 in Pseudomonas aeruginosa strain PAO.

作者信息

Jarrell K, Kropinski A M

出版信息

J Virol. 1977 Sep;23(3):461-6. doi: 10.1128/JVI.23.3.461-466.1977.

Abstract

Bacteriophage E79 was shown to interact with the lipopolysaccharide (LPS) of Pseudomonas aeruginosa strain PAO. LPS isolated from an E79-sensitive, smooth strain inactivated the phage, exhibiting a Phl50 value (concentration of LPS that caused a 50% decrease in the titer of phage during 1 h of incubation at 37 degrees C) of 0.04 microgram/ml, whereas the LPS isolated from a rough mutant derived from the wild type showed no neutralizing activity towards E79. EDTA and sodium deoxycholate were demonstrated to abolish the neutralizing capacity of the smooth LPS. One E79 receptor site was shown to be equivalent to 10(-16) g of LPS.

摘要

噬菌体E79被证明可与铜绿假单胞菌PAO菌株的脂多糖(LPS)相互作用。从对E79敏感的光滑菌株中分离出的LPS可使噬菌体失活,其Phl50值(在37℃孵育1小时期间导致噬菌体滴度降低50%的LPS浓度)为0.04微克/毫升,而从野生型衍生的粗糙突变体中分离出的LPS对E79没有中和活性。已证明EDTA和脱氧胆酸钠可消除光滑LPS的中和能力。一个E79受体位点相当于10^(-16)克LPS。

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本文引用的文献

1
Pseudomonas genetics.假单胞菌遗传学
Annu Rev Genet. 1971;5:425-46. doi: 10.1146/annurev.ge.05.120171.002233.
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[Isolation of bacteriophage receptor substances in Shigella].
Ann Inst Pasteur (Paris). 1960 Jun;98:910-4.
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Lysogeny in Pseudomonas aeruginosa.铜绿假单胞菌中的溶原现象。
Aust J Exp Biol Med Sci. 1960 Aug;38:321-9. doi: 10.1038/icb.1960.34.
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Bacterial viruses; with particular reference to adsorption/penetration.细菌病毒;特别涉及吸附/穿透
Annu Rev Microbiol. 1958;12:27-48. doi: 10.1146/annurev.mi.12.100158.000331.

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