Veale D R, Penn C W, Sutton S, Smith H
J Gen Microbiol. 1978 May;106(1):129-36. doi: 10.1099/00221287-106-1-129.
The high natural resistance of gonococci showing a characteristic 'double highlight' (DH) colonial morphology (Penn, Veale & Smith, 1977b) to intracellular killing by human phagocytes was markedly reduced by addition of rabbit antiserum to the phagocytosis medium or by preincubation of organisms with antiserum. Antisera raised to three different DH gonococcal strains showed a complex pattern of specificity in phagocytosis tests with the homologous organisms and three other DH strains. The effect of antiserum could be neutralized by adsorption with intact organisms or with extracts, prepared ultrasonically, of the homologous strain. Antiserum also promoted the intracellular killing of a strain which had a 'single highlight' colonial morphology (Penn et al., 1977b) and a low natural resistance to phagocytic killing, but adsorption with this strain neutralized the antiserum less consistently than the DH strain. The neutralization of antiserum-mediated promotion of intracellular killing by extracts of organisms naturally resistant to such killing may provide an assay for the aggressins responsible for this resistance.
表现出特征性“双亮点”（DH）菌落形态的淋球菌（Penn、Veale和Smith，1977b）对人类吞噬细胞的细胞内杀伤具有很高的天然抗性，通过在吞噬培养基中添加兔抗血清或使细菌与抗血清预孵育，这种抗性会显著降低。针对三种不同DH淋球菌菌株产生的抗血清，在对同源菌株和其他三种DH菌株进行的吞噬试验中显示出复杂的特异性模式。抗血清的作用可以通过用完整细菌或用同源菌株超声制备的提取物吸附来中和。抗血清还促进了具有“单亮点”菌落形态（Penn等人，1977b）且对吞噬杀伤天然抗性较低的菌株的细胞内杀伤，但用该菌株吸附中和抗血清的效果不如DH菌株一致。对天然抗吞噬杀伤的细菌提取物介导的抗血清促进细胞内杀伤作用的中和，可能为负责这种抗性的侵袭素提供一种检测方法。
