Penn C W, Veale D R, Smith H
J Gen Microbiol. 1977 May;100(1):147-58. doi: 10.1099/00221287-100-1-147.
Gonococci from subcutaneously implanted chambers in guinea pigs produced, on agar, more than 95% small colonies showing a "double highlight" (DH) effect in oblique reflected light combined with transmitted light. Laboratory strains of gonococci produced some DH colonies, but other showed a single highlight (SH) or no highlight (NH). Selection of DH colonies and comparison of their organisms with gonococci grown in vivo and with those from SH colonies, showed that the DH character was associated with high infectivity for guinea-pig chambers, resistance to killing by human phagocytes and heavy pilation. Furthermore, DH colonies were found in the first culture of three fresh samples of urethral pus. Thus, the DH colony characteristic may be a more reliable criterion of pathogenicity of gonococcal isolates than systems used previously. There were, however, some differences between the gonococci grown in vivo and the DH colony types. The gonococci grown in vivo and cultured once on solid medium possessed one or two antigens which differed from those of DH (or SH) colonies. They also formed smooth suspensions (which separated slowly) in saline, compared with the rough suspensions (which separated quickly) formed by gonococci from DH (or SH) colonies. Finally, the organisms grown in vivo were resistant to killing by human serum whereas the DH (and SH) colony types were susceptible; the resistance of the organisms grown in vivo was lost during one subculture on agar suggesting that the property is a phenotypic characteristic. Hence, in addition to selecting DH colony types the conditions in vivo produce organisms which differ, probably phenotypically, from cultured organisms.
在豚鼠皮下植入腔室中培养出的淋球菌，在琼脂平板上产生了超过95%的小菌落，这些菌落在斜射反射光与透射光相结合的情况下呈现出“双重高光”（DH）效应。淋球菌的实验室菌株产生了一些DH菌落，但其他的则呈现单一高光（SH）或无高光（NH）。挑选出DH菌落，并将其菌株与在体内生长的淋球菌以及来自SH菌落的菌株进行比较，结果表明，DH特性与对豚鼠腔室的高感染性、对人类吞噬细胞杀伤作用的抗性以及大量菌毛形成有关。此外，在三份新鲜尿道脓性样本的初次培养物中发现了DH菌落。因此，与之前使用的系统相比，DH菌落特征可能是淋球菌分离株致病性的更可靠标准。然而，在体内生长的淋球菌与DH菌落类型之间存在一些差异。在体内生长并在固体培养基上培养一次的淋球菌具有一两种与DH（或SH）菌落不同的抗原。与来自DH（或SH）菌落的淋球菌形成的粗糙悬液（快速分离）相比，它们在盐水中形成的是光滑悬液（分离缓慢）。最后，在体内生长的菌株对人血清的杀伤作用具有抗性，而DH（和SH）菌落类型则敏感；在琼脂上进行一次传代培养后，体内生长的菌株的这种抗性丧失了，这表明该特性是一种表型特征。因此，除了挑选DH菌落类型外，体内条件产生的菌株可能在表型上与培养的菌株不同。
