pH调节海胆卵皮质中肌动蛋白的聚合。
pH regulates the polymerization of actin in the sea urchin egg cortex.
作者信息
Begg D A, Rebhun L I
出版信息
J Cell Biol. 1979 Oct;83(1):241-8. doi: 10.1083/jcb.83.1.241.
Abstract
The state of actin in the isolated cortex of the unfertilized sea urchin egg can be controlled by experimentally manipulating the pH of the isolation medium. Cortices isolated at the pH of the unfertilized egg (6.5--6.7) do not contain filamentous actin, while those isolated at the pH of the fertilized egg (7.3--7.5) develop large numbers of microvilli which contain bundles of actin filaments. Cortices that are isolated at pH 6.5 and then transferred to isolation medium buffered at pH 7.5 also develop actin filaments. However, the filaments are not arranged in bundles and microvilli do not form. Although the cortical granules in cortices isolated at pH 6.5 discharge at a free Ca++ concentration of approximately 10 micrometer, actin polymerization is not induced by increasing the Ca++ concentration of the isolation medium. These results suggest that the increase in cytoplasmic pH which occurs following fertilization induces the polymerization of actin in the egg cortex.
摘要
通过实验控制未受精海胆卵分离皮层中肌动蛋白的状态，可以通过操纵分离介质的pH值来实现。在未受精卵的pH值（6.5 - 6.7）下分离的皮层不含有丝状肌动蛋白，而在受精卵的pH值（7.3 - 7.5）下分离的皮层则会形成大量含有肌动蛋白丝束的微绒毛。在pH 6.5下分离然后转移到pH 7.5缓冲的分离介质中的皮层也会形成肌动蛋白丝。然而，这些丝不是成束排列的，微绒毛也不会形成。尽管在pH 6.5下分离的皮层中的皮质颗粒在游离Ca++浓度约为10微米时会释放，但增加分离介质中的Ca++浓度并不会诱导肌动蛋白聚合。这些结果表明，受精后细胞质pH值的升高会诱导卵皮层中肌动蛋白的聚合
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