Bankhurst A D, Warner N L, Sprent J
J Exp Med. 1971 Oct 1;134(4):1005-15. doi: 10.1084/jem.134.4.1005.
Lymphoid cells from thymus, thoracic duct lymph (TDL), and thoracic duct lymph in irradiated animals reconstituted with allogeneic thymus cells (TTDL) were labeled with radioiodinated anti-immunoglobulins using radioautographic techniques. Thymus and TTDL were labeled (14.4 and 37.0%, respectively) with anti-light chain protein after prolonged exposures (30-60 days). No labeling was observed on thymus and TTDL with anti-polyheavy chain globulin. In contrast 18.5-19.0% of TDL labeled on short exposure (6 days) with anti-polyheavy chain and anti-light chain materials. It is proposed that the difference between the labeling observed on short exposures versus long exposures can be related to the difference in surface density of immunoglobulins between nonthymus-derived (B) and thymus-derived (T) cells. The distribution of labeled cells in the thymus was preferentially among the larger cells (greater than 10 micro diameter). The TTDL population was mostly composed of a larger, blast-like population and the distribution of label was independent of size. As the thymus and TTDL preparations contain almost exclusively T cells, this represents a direct demonstration of surface immunoglobulin light chains on T lymphoid cells.
利用放射自显影技术，用放射性碘化抗免疫球蛋白标记来自胸腺、胸导管淋巴（TDL）以及用同种异体胸腺细胞重建的经辐射动物的胸导管淋巴（TTDL）中的淋巴细胞。长时间暴露（30 - 60天）后，胸腺和TTDL分别被抗轻链蛋白标记（分别为14.4%和37.0%）。用抗多聚重链球蛋白未观察到胸腺和TTDL有标记。相比之下，18.5 - 19.0%的TDL在短时间暴露（6天）时被抗多聚重链和抗轻链物质标记。有人提出，短时间暴露与长时间暴露时观察到的标记差异可能与非胸腺来源（B）细胞和胸腺来源（T）细胞之间免疫球蛋白表面密度的差异有关。胸腺中标记细胞的分布优先在较大的细胞（直径大于10微米）中。TTDL群体主要由较大的、母细胞样群体组成，标记的分布与大小无关。由于胸腺和TTDL制剂几乎只含有T细胞，这直接证明了T淋巴细胞表面存在免疫球蛋白轻链。
