4-硝基喹啉 1-氧化物与四种脱氧核糖核苷酸的相互作用。
Interactions of 4-nitroquinoline 1-oxide with four deoxyribonucleotides.
作者信息
Winkle S A, Tinoco I
出版信息
Biochemistry. 1978 Apr 4;17(7):1352-6. doi: 10.1021/bi00600a033.
The interactions of 4-nitroquinoline 1-oxide (NQO) with the four 5'-deoxyribonucleotides were probed using absorption spectra of the charge transfer bands and 1H and 13C nuclear magnetic resonance (NMR) spectra of nucleotide-NQO mixtures. Spectral data yielded equilibrium constants (K(dpG:NQO) = 16 M-1, K(dpA:NQO) = 12 M-1, K(dpT:NQO) = K(dpC:NQO) = 4 M-1) which suggest the preference of NQO for the guanine residue in a DNA. This is in agreement with the data of Okano, T., et al. [(1969) Gann 60, 295]. From 13C and 1H NMR data on nucleosides, a structure for the dpG:NQO complex is proposed.
利用电荷转移带的吸收光谱以及核苷酸 - NQO混合物的1H和13C核磁共振（NMR）光谱，研究了4 - 硝基喹啉1 - 氧化物（NQO）与四种5'-脱氧核糖核苷酸的相互作用。光谱数据得出了平衡常数（K(dpG:NQO) = 16 M-1，K(dpA:NQO) = 12 M-1，K(dpT:NQO) = K(dpC:NQO) = 4 M-1），这表明NQO在DNA中更倾向于与鸟嘌呤残基结合。这与冈野敏郎等人的数据一致[（1969年）《癌》60, 295]。根据核苷的13C和1H NMR数据，提出了dpG:NQO复合物的结构。
Zotero 插件