Aparicio S R, Marsden P
J Clin Pathol. 1969 Sep;22(5):589-92. doi: 10.1136/jcp.22.5.589.
Staining of semi-thin sections of osmium-fixed epoxy resin (Epon or Araldite)-embedded tissues with haematoxylin and acid dyes as counterstains (eosin or phloxin) was successfully achieved after intense oxidation using hydrogen peroxide as the oxygen donor. Results are closely similar to those obtained with paraffin sections (haematoxylin and eosin): nuclei stain blue, cytoplasm and extracellular structures (eg, collagen, elastica, basement membranes) stain with varying shades of red. Preliminary studies show that similarly good results, comparable with standard paraffinembedded histological materials, can be obtained, using H(2)O(2) pretreatment, with Weigert's iron haematoxylin, Mallory's phosphotungstic acid haematoxylin, Alcian blue, PAS reaction, Sudan III, Sudan black, oil red, Gomori's aldehyde fuchsin, and Baker's acid haematein.
以过氧化氢作为氧供体进行强烈氧化后，成功实现了用苏木精和酸性染料（伊红或焰红）对锇固定的环氧树脂（Epon或Araldite）包埋组织的半薄切片进行染色作为复染。结果与石蜡切片（苏木精和伊红）获得的结果非常相似：细胞核染成蓝色，细胞质和细胞外结构（如胶原蛋白、弹性蛋白、基底膜）染成不同深浅的红色。初步研究表明，使用H₂O₂预处理，用魏格特铁苏木精、马洛里磷钨酸苏木精、阿尔辛蓝、PAS反应、苏丹III、苏丹黑、油红、戈莫里醛品红和贝克酸性苏木精，可获得与标准石蜡包埋组织学材料相当的同样良好的结果。
