一种用铁蛋白 - 抗体偶联物对细胞内抗原进行特异性染色的通用方法。
A general method for the specific staining of intracellular antigens with ferritin-antibody conjugates.
作者信息
McLean J D, Singer S J
出版信息
Proc Natl Acad Sci U S A. 1970 Jan;65(1):122-8. doi: 10.1073/pnas.65.1.122.
Abstract
A general method is described whereby an intracellular macromolecule can be specifically stained with its antibody conjugated to ferritin. In this method cells or cell organelles are fixed, embedded in bovine serum albumin cross-linked with glutaraldehyde or formaldehyde, and then sectioned. This procedure preserves antigenic determinants as well as the cellular ultrastructure. The application of the ferritin-antibody conjugate to the section produces specific staining of the exposed antigen.
摘要
本文描述了一种通用方法，通过该方法可以用与铁蛋白偶联的抗体对细胞内大分子进行特异性染色。在该方法中，细胞或细胞器被固定，包埋于用戊二醛或甲醛交联的牛血清白蛋白中，然后进行切片。该程序既能保留抗原决定簇，又能保留细胞超微结构。将铁蛋白-抗体偶联物应用于切片会使暴露的抗原产生特异性染色。
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