Clouston J G, Wills P A
J Bacteriol. 1970 Jul;103(1):140-3. doi: 10.1128/jb.103.1.140-143.1970.
The kinetics of initiation of germination and inactivation by hydrostatic pressure of phosphate-buffered Bacillus pumilus spores is shown to be a consecutive first-order process at 25 C. The effect of increasing pressure at constant temperature was studied, and rate constants were derived by using the criteria of heat resistance, refractility, and stainability. The calculated volume change of activation (DeltaVdouble dagger) was -139 +/- 6 cm(3)/mole for loss of heat resistance, -158 +/- 8 cm(3)/mole for the loss of refractility, and -153 +/- 4 cm(3)/mole for the change in permeability to dilute stains for the pressure range 800 to 1,010 atm at 25 C. It is suggested that the spore exists as a Donnan phase and that pressure triggers germination by influencing the equilibrium.
在25℃下，磷酸盐缓冲的短小芽孢杆菌孢子的发芽起始动力学和静水压失活动力学显示为连续一级过程。研究了在恒定温度下增加压力的影响，并通过使用耐热性、折光性和可染性标准得出速率常数。在25℃下800至1010个大气压的压力范围内，计算得出的活化体积变化（ΔV‡）对于耐热性丧失为-139±6 cm³/摩尔，对于折光性丧失为-158±8 cm³/摩尔，对于对稀释染料的渗透性变化为-153±4 cm³/摩尔。有人提出，孢子以唐南相存在，压力通过影响平衡触发发芽。
