Abdou N I, Richter M
Immunology. 1970 Jun;18(6):833-41.
The antigenicity of native, aggregate-free and heat-aggregated HGG was investigated and , using the cell culture and fragment culture systems. Although the aggregate-free material displayed very low immunogenicity , its antigenicity was markedly enhanced if injected along with Freund's adjuvant. Results of cell culture experiments, using immune spleen cells, paralleled the results in that the aggregated HGG was highly blastogenic whereas the aggregate-free HGG could not induce blastogenesis of the immune cells. Results of an opposite nature derived from the fragment culture experiments in that aggregate-free HGG could readily induce a secondary immune response whereas the aggregated HGG was unable to do so. The significance of these results is discussed.
利用细胞培养和片段培养系统，对天然、无聚集体和热聚集的人γ球蛋白（HGG）的抗原性进行了研究。尽管无聚集体的物质显示出非常低的免疫原性，但如果与弗氏佐剂一起注射，其抗原性会显著增强。使用免疫脾细胞进行的细胞培养实验结果与之相似，即聚集的HGG具有高度的致有丝分裂原性，而无聚集体的HGG不能诱导免疫细胞的有丝分裂。片段培养实验得出了相反性质的结果，即无聚集体的HGG能够轻易诱导二次免疫反应，而聚集的HGG则不能。讨论了这些结果的意义。
