反应性溶解：补体介导的未致敏细胞溶解。I. 指示因子的特性及其鉴定为C7。
Reactive lysis: the complement-mediated lysis of unsensitized cells. I. The characterization of the indicator factor and its identification as C7.
作者信息
Thompson R A, Lachmann P J
出版信息
J Exp Med. 1970 Apr 1;131(4):629-41. doi: 10.1084/jem.131.4.629.
Abstract
This paper describes the characteristics of the indicator factor (I) which takes part in reactive hemolysis and its identification as the seventh component of complement. I was shown to be a beta globulin with a sediment coefficient of 5.7S and a molecular weight of about 140,000. Experiments on the depletion of I activity with anti-I antiserum or with activated R euglobulin showed that I was a late acting complement component necessary for the lysis of cells after the EAC142 stage. Complement component analysis of purified I fractions excluded all known components except C7. The physicochemical characteristics of I are compatible with published data on C7. The method of quantitation described represents a convenient method of testing for C7.
摘要
本文描述了参与反应性溶血的指示因子（I）的特性，并将其鉴定为补体的第七成分。已证明I是一种β球蛋白，沉降系数为5.7S，分子量约为140,000。用抗I抗血清或活化的R优球蛋白耗尽I活性的实验表明，I是EAC142阶段后细胞裂解所必需的晚期补体成分。对纯化的I组分进行补体成分分析，排除了除C7之外的所有已知成分。I的物理化学特性与已发表的关于C7的数据相符。所描述的定量方法是一种检测C7的简便方法。
相似文献
1
Reactive lysis: the complement-mediated lysis of unsensitized cells. I. The characterization of the indicator factor and its identification as C7.反应性溶解：补体介导的未致敏细胞溶解。I. 指示因子的特性及其鉴定为C7。
J Exp Med. 1970 Apr 1;131(4):629-41. doi: 10.1084/jem.131.4.629.
2
Reactive lysis: the complement-mediated lysis of unsensitized cells. II. The characterization of activated reactor as C56 and the participation of C8 and C9.反应性溶解：补体介导的未致敏细胞溶解。II. 活化反应体表征为C56以及C8和C9的参与。
J Exp Med. 1970 Apr 1;131(4):643-57. doi: 10.1084/jem.131.4.643.
3
4
Hemolysis of normal human erythrocytes by autologous serum complement.自体血清补体对正常人红细胞的溶血作用。
Int Arch Allergy Immunol. 1993;100(3):209-14. doi: 10.1159/000236413.
5
[The C1-inactivator of guinea pig serum: titration, purification and characterization of the inactivator].[豚鼠血清的C1灭活剂：灭活剂的滴定、纯化及特性鉴定]
Z Med Mikrobiol Immunol. 1971;156(2):194-207.
6
Rat 7S immunoglobulins: characterization of gamma-2- and gamma-1-anti-haptens antibodies.大鼠7S免疫球蛋白：γ-2和γ-1抗半抗原抗体的特性
Immunology. 1969 May;16(5):589-99.
7
The first component of guinea pig complement: hemolytic assays with EA, EAC4 and with rat and guinea pig late acting components.豚鼠补体的第一成分：用EA、EAC4以及大鼠和豚鼠后期作用成分进行溶血试验。
Immunochemistry. 1971 Jun;8(6):517-23. doi: 10.1016/0019-2791(71)90403-4.
8
9
The activation of Cls with purified Clr.用纯化的Clr激活Cls。
Immunochemistry. 1974 Apr;11(4):191-6. doi: 10.1016/0019-2791(74)90327-9.
10
Isolation of the sixth component of complement from human serum.从人血清中分离补体第六成分。
Immunochemistry. 1971 Nov;8(11):995-1006. doi: 10.1016/0019-2791(71)90487-3.
引用本文的文献
1
Complement C7 and clusterin form a complex in circulation.补体 C7 与簇集素在循环中形成复合物。
Front Immunol. 2024 Jan 25;15:1330095. doi: 10.3389/fimmu.2024.1330095. eCollection 2024.
2
As in Real Estate, Location Matters: Cellular Expression of Complement Varies Between Macular and Peripheral Regions of the Retina and Supporting Tissues.与房地产一样，位置很重要：视网膜和支持组织的黄斑区和周边区域的补体表达存在差异。
Front Immunol. 2022 Jun 15;13:895519. doi: 10.3389/fimmu.2022.895519. eCollection 2022.
3
A Personal Tribute to a Highly Inspiring Mentor, Professor Sir Peter J. Lachmann, 1931-2020.向极具启发性的导师彼得·J·拉赫曼爵士教授致敬，1931-2020 年。
Viruses. 2021 Jan 29;13(2):206. doi: 10.3390/v13020206.
4
Bacterial killing by complement requires direct anchoring of membrane attack complex precursor C5b-7.补体杀菌需要直接锚定膜攻击复合物前体 C5b-7。
PLoS Pathog. 2020 Jun 22;16(6):e1008606. doi: 10.1371/journal.ppat.1008606. eCollection 2020 Jun.
5
Complement-mediated 'bystander' damage initiates host NLRP3 inflammasome activation.补体介导的“旁观者”损伤引发宿主NLRP3炎性小体激活。
J Cell Sci. 2016 May 1;129(9):1928-39. doi: 10.1242/jcs.179291. Epub 2016 Mar 22.
6
Circulating Immune Complex Levels are Associated with Disease Severity and Seasonality in Children with Malaria from Mali.循环免疫复合物水平与来自马里的疟疾患儿的疾病严重程度和季节性相关。
Biomark Insights. 2012;7:81-6. doi: 10.4137/BMI.S9624. Epub 2012 Jul 3.
7
Molecular characterization of the alpha subunit of complement component C8 (GcC8alpha) in the nurse shark (Ginglymostoma cirratum).护士鲨（Ginglymostoma cirratum）中补体成分C8（GcC8α）α亚基的分子特征分析
Fish Shellfish Immunol. 2009 Sep;27(3):397-406. doi: 10.1016/j.fsi.2009.05.020. Epub 2009 Jun 12.
8
Use of a genetic isolate to identify rare disease variants: C7 on 5p associated with MS.利用遗传隔离群体鉴定罕见病变异：5号染色体短臂上的C7与多发性硬化症相关。
Hum Mol Genet. 2009 May 1;18(9):1670-83. doi: 10.1093/hmg/ddp073. Epub 2009 Feb 16.
9
Deficiencies of the complement MAC II gene cluster (C6, C7, C9): is subtotal C6 deficiency of particular evolutionary benefit?补体膜攻击复合物II基因簇（C6、C7、C9）缺陷：C6部分缺陷是否具有特殊的进化优势？
Clin Exp Immunol. 2003 Aug;133(2):156-9. doi: 10.1046/j.1365-2249.2003.02230.x.
10
Simple method to distinguish between primary and secondary C3 deficiencies.区分原发性和继发性C3缺乏症的简单方法。
Clin Diagn Lab Immunol. 2003 Mar;10(2):216-20. doi: 10.1128/cdli.10.2.216-220.2003.
本文引用的文献
1
Assay and properties of serum inhibitor of C'l-esterase.C1酯酶血清抑制剂的测定与特性
Proc Soc Exp Biol Med. 1959 Aug-Sep;101:608-11. doi: 10.3181/00379727-101-25034.
2
3
Methods for the separation, purification and measurement of nine components of hemolytic complement in guinea-pig serum.豚鼠血清中溶血补体九种成分的分离、纯化及测定方法
Immunochemistry. 1966 Mar;3(2):111-35. doi: 10.1016/0019-2791(66)90292-8.
4
5
6
Deficiency of the fifth component of complement in mice with an inherited complement defect.具有遗传性补体缺陷的小鼠中补体第五成分的缺乏。
J Exp Med. 1967 Jan 1;125(1):1-16. doi: 10.1084/jem.125.1.1.
7
Immunochemical quantitation of antigens by single radial immunodiffusion.通过单向辐射免疫扩散法对抗原进行免疫化学定量。
Immunochemistry. 1965 Sep;2(3):235-54. doi: 10.1016/0019-2791(65)90004-2.
8
Reactive lysis: the complement-mediated lysis of unsensitized cells. II. The characterization of activated reactor as C56 and the participation of C8 and C9.反应性溶解：补体介导的未致敏细胞溶解。II. 活化反应体表征为C56以及C8和C9的参与。
J Exp Med. 1970 Apr 1;131(4):643-57. doi: 10.1084/jem.131.4.643.
9
Reactive haemolysis--a distinctive form of red cell lysis.反应性溶血——一种独特的红细胞溶解形式。
Immunology. 1968 May;14(5):745-62.
10
Complement: substitution of the terminal component in immune hemolysis by 1,10-phenanthroline.补体：用1,10 - 菲咯啉替代免疫溶血中的末端成分。
Science. 1967 Jul 28;157(3787):442-3. doi: 10.1126/science.157.3787.442.
Zotero 插件