Glatz B A, Goeppert J M
Infect Immun. 1973 Jul;8(1):25-9. doi: 10.1128/iai.8.1.25-29.1973.
A guinea pig skin assay was developed as a measure of ileal loop fluid-inducing factor in the rabbit and possibly of the causative principle of Bacillus cereus food poisoning. A green or bloody necrotic reaction was produced in the guinea pig skin by injection of culture filtrates from 21 of 24 B. cereus strains tested, and by no other Bacillus species tested except for the closely related B. thuringiensis. The skin factor was synthesized and excreted by logarithmically growing cells, inactivated by heating at 56 C for 5 min, precipitable by ammonium sulfate, and was nondialyzable. The skin activity was not related to the lecithinolytic or hemolytic activities of B. cereus. Production of the skin factor depended on the medium in which the culture was growing. The factor was an antigenically active substance. There are indications that at least two antigenically distinct species of skin factors exist.
已开发出一种豚鼠皮肤试验，用于检测兔回肠袢液诱导因子，也可能用于检测蜡样芽孢杆菌食物中毒的致病原理。在所测试的24株蜡样芽孢杆菌中，有21株的培养滤液注射到豚鼠皮肤后会产生绿色或血性坏死反应，除密切相关的苏云金芽孢杆菌外，其他测试的芽孢杆菌物种均未产生此反应。皮肤因子由对数生长期的细胞合成并分泌，在56℃加热5分钟会失活，可被硫酸铵沉淀，且不可透析。皮肤活性与蜡样芽孢杆菌的卵磷脂分解或溶血活性无关。皮肤因子的产生取决于培养所用的培养基。该因子是一种具有抗原活性的物质。有迹象表明至少存在两种抗原性不同的皮肤因子。
