通过免疫荧光法在细胞培养中早期检测流感病毒。
Early detection of influenza virus in cell culture by means of immunofluorescence.
作者信息
Rahman A F
出版信息
J Clin Pathol. 1973 Jul;26(7):503-5. doi: 10.1136/jcp.26.7.503.
Abstract
An indirect fluorescent antibody technique using human serum for the rapid detection of influenza A virus in monkey kidney cell cultures is described. Influenza A2 virus was isolated from 38 of 88 nose/throat swabs received from patients with suspected influenza during the winter of 1971-72.Thirty-two strains isolated in monkey kidney cell cultures were identified by immunofluorescence on the day haemadsorption was observed, 22 of them within a week of receipt of the specimen. Human antiserum was found to be very satisfactory for this purpose.
摘要
本文描述了一种使用人血清的间接荧光抗体技术，用于在猴肾细胞培养物中快速检测甲型流感病毒。在1971 - 1972年冬季，从88份疑似流感患者的鼻/咽拭子中，有38份分离出了甲型流感病毒。在观察到血细胞吸附的当天，通过免疫荧光法对在猴肾细胞培养物中分离出的32株病毒进行了鉴定，其中22株是在收到标本后的一周内鉴定的。发现人抗血清用于此目的非常令人满意。
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