Bye A, Land G
J Chromatogr. 1975 Dec 10;115(1):93-9. doi: 10.1016/s0021-9673(00)89020-2.
A gas chromatographic method is described for assay of 3-(5-tetrazolyl) thioxanthone 10,10-dioxide (BW 59C) in human plasma, urine and faeces. After extraction into 1,2-dichloroethane from alkaline medium the compound is converted to the heptafluorobutyrate derivative which is injected into a gas chromatograph and measured using a 63Ni electron capture detector. The assay produces a linear calibration curve over the range 0-30 mug/ml when the internal standard method is used. Reproducibility is good and sensitivity down to 1 ng injected on column is possible. The method has been used to investigate the pharmacokinetic properties of BW 59C in man and has been semi-automated by the use of an autosampler and dedicated computer.
本文描述了一种气相色谱法，用于测定人血浆、尿液和粪便中的3-(5-四唑基)噻吨酮10,10-二氧化物（BW 59C）。在碱性介质中用1,2-二氯乙烷萃取后，该化合物转化为七氟丁酸酯衍生物，注入气相色谱仪并用63Ni电子捕获检测器进行测定。使用内标法时，该测定法在0-30μg/ml范围内产生线性校准曲线。重现性良好，柱上进样量低至1 ng时也具有灵敏度。该方法已用于研究BW 59C在人体中的药代动力学特性，并通过使用自动进样器和专用计算机实现了半自动化。
