Carlsson J, Collins P, Brunk U
Acta Pathol Microbiol Scand A. 1978 Jan;86(1):45-55. doi: 10.1111/j.1699-0463.1978.tb02011.x.
Human glioma cells, growing as spherical colonies in agarose gel, or as monolayers on glass or plastic, were studied with time-lapse cinematography and electron microscopy. The cells in the agarose cultured colonies often had ruffling-like membrane structures which were similar in for, although smaller in size, than those observed on monolayer cultured cells. The ruffling-like structures were more frequent at the periphery than in the central regions of the colonies which was in parallel to the proliferative pattern. In time-lapse cinematography it was seen that pinocytotic vacuoles were formed from ruffling membranes in the monolayer cultures. In the transmission electron microscope, such vacuoles were also found near the ruffling-like structures in the agarose cultured cells. In dense monolayer cultures, ruffling and associated pinocytosis were to a large extent transferred from the margin to the upper surface of the cells. This capacity may be an important property for the ability of the malignant cells to attain extreme high cell densities in monolayer cultures and to grow as colonies in agarose cultures. As has been previously shown, the normal counterpart of the gliomas, the glia cells, cannot grow to high densities; they do not ruffle on their upper cell surface and are unable to grow in suspension or agarose culture.
利用延时摄影和电子显微镜对人胶质瘤细胞进行了研究，这些细胞在琼脂糖凝胶中呈球形集落生长，或在玻璃或塑料上呈单层生长。琼脂糖培养集落中的细胞通常具有类似波纹的膜结构，其形态与单层培养细胞上观察到的相似，只是尺寸较小。类似波纹的结构在集落周边比在中央区域更频繁，这与增殖模式平行。在延时摄影中可以看到，单层培养中由波纹膜形成了胞饮泡。在透射电子显微镜下，在琼脂糖培养细胞的类似波纹的结构附近也发现了这样的泡。在密集的单层培养中，波纹和相关的胞饮作用在很大程度上从细胞边缘转移到了细胞上表面。这种能力可能是恶性细胞在单层培养中达到极高细胞密度并在琼脂糖培养中形成集落的能力的一个重要特性。如先前所示，胶质瘤的正常对应物神经胶质细胞不能生长到高密度；它们在上细胞表面不会形成波纹，也不能在悬浮或琼脂糖培养中生长。
