Holstein B
Acta Physiol Scand. 1979 Jan;105(1):93-107. doi: 10.1111/j.1748-1716.1979.tb06317.x.
Gastric acid secretion was measured in unanaesthetized codfish surgically equipped with a catheter draining the stomach. The pylorus was ligated. Without perfusion of the intestine, or during perfusion with pure sea water (100% SW) or slightly diluted (67%) SW, gastric acid secretion was low ( less than 10 mumol H+/kg.h) and the fishes became dehydrated during the experiment. Perfusion of the intestine with 50% or 3% SW enabled the fishes to compensate for the water loss to the environment and greatly enhanced gastric acid secretion. Acid secretion was elevated also in fishes in which the dehydration was prevented by an intra-muscular infusion of saline. Perfusion of the intestine (8.5 ml/h) decreased the hourly effluent volume ('drinking rate') from the stomach to almost zero within 2 h. The effect was independent of the degree of dilution of the perfusing SW, but was less pronounced when the perfusion rate was lowered. Intra-muscular saline also depressed 'drinking rate'. The acid secretion prevailing during perfusion of the intestine with SW at a dilution preventing dehydration--suggested to represent the basal acid secretion of the codfish--was inhibited by atropine, hexamethonium, and metiamide.
在通过手术安装了用于引流胃的导管的未麻醉鳕鱼中测量胃酸分泌。幽门被结扎。在不灌注肠道的情况下，或在用纯海水（100% SW）或轻度稀释（67%）的SW灌注期间，胃酸分泌较低（小于10 μmol H⁺/kg·h），并且鱼在实验过程中会脱水。用50%或3%的SW灌注肠道能使鱼补偿向环境中的水分流失，并极大地增强胃酸分泌。通过肌肉注射生理盐水防止脱水的鱼中，胃酸分泌也会升高。以8.5 ml/h的速度灌注肠道会使胃的每小时流出量（“饮水率”）在2小时内降至几乎为零。该效果与灌注SW的稀释程度无关，但当灌注速率降低时，效果不太明显。肌肉注射生理盐水也会降低“饮水率”。在用防止脱水的稀释度的SW灌注肠道期间普遍存在的胃酸分泌——被认为代表鳕鱼的基础胃酸分泌——会被阿托品、六甲铵和甲硫咪特抑制。
