Dhital Subhash, Deo Pankaj, Bharathwaj Manasa, Horan Kristy, Nickson Joshua, Azad Mohammad, Stuart Isabella, Chow Seong H, Gunasinghe Sachith D, Bamert Rebecca, Li Jian, Lithgow Trevor, Howden Benjamin P, Naderer Thomas
Infection Program, Monash Biomedicine Discovery Institute and Department of Biochemistry and Molecular Biology, Monash University, Clayton, Australia.
Centre to Impact AMR, Monash University, Clayton, Australia.
Microlife. 2022 Jul 29;3:uqac013. doi: 10.1093/femsml/uqac013. eCollection 2022.
causes the sexually transmitted disease gonorrhoea. The treatment of gonorrhoea is becoming increasingly challenging, as has developed resistance to antimicrobial agents routinely used in the clinic. Resistance to penicillin is wide-spread partly due to the acquisition of β-lactamase genes. How survives an initial exposure to β-lactams before acquiring resistance genes remains to be understood. Here, using a panel of clinical isolates of we show that the β-lactamase enzyme is packaged into outer membrane vesicles (OMVs) by strains expressing or , which protects otherwise susceptible clinical isolates from the β-lactam drug amoxycillin. We characterized the phenotypes of these clinical isolates of and the time courses over which the cross-protection of the strains is effective. Imaging and biochemical assays suggest that OMVs promote the transfer of proteins and lipids between bacteria. Thus, strains secret antibiotic degrading enzymes via OMVs enabling survival of otherwise susceptible bacteria.
引起性传播疾病淋病。淋病的治疗正变得越来越具有挑战性，因为其已对临床常规使用的抗菌药物产生了耐药性。对青霉素的耐药性广泛存在，部分原因是获得了β-内酰胺酶基因。在获得耐药基因之前，它如何在初次接触β-内酰胺类药物后存活下来仍有待了解。在这里，我们使用一组淋病奈瑟菌临床分离株表明，β-内酰胺酶被表达或的菌株包装到外膜囊泡（OMV）中，这保护了原本易感的临床分离株免受β-内酰胺类药物阿莫西林的影响。我们对这些淋病奈瑟菌临床分离株的表型以及菌株交叉保护有效的时间进程进行了表征。成像和生化分析表明，OMV促进了细菌之间蛋白质和脂质的转移。因此，淋病奈瑟菌菌株通过OMV分泌抗生素降解酶，使原本易感的细菌得以存活。
