Carifo K, Catlin B W
Appl Microbiol. 1973 Sep;26(3):223-30. doi: 10.1128/am.26.3.223-230.1973.
A system is described for differentiating clinïcal isolates of Neisseria gonorrhoeae based on their growth or absence of growth on a set of 11 chemically defined agar media. The complete medium, NEDA, contains all of the compounds required for gonococcal growth; but isolates differ in their ability to grow on NEDA from which selected compounds are individually omitted. The differential compounds include L-proline, L-arginine, L-ornithine, L-methionine, hypoxanthine, uracil, thiamine, and thiamine pyrophosphate. A distinctive pattern of growth responses on the standard media defines an auxotype. Twenty auxotypes were found among a group of 251 gonococci which were isolated from patients examined in the clinics of one city during a 3-month span of time. Another collection of 74 strains from several different countries yielded two additional auxotypes. The stability of the nutritional requirements on which the auxotyping depends was verified in two ways. Cultures isolated from different anatomic sites of a patient or from sexual partners represented the same auxotype, as did cultures which were repeatedly isolated from cases of presumptive treatment failures. Also, the auxotypes of gonococci remained the same after numerous subcultures. The reproducibility of results and the variety and number of auxotypes indicate the potential value of the auxotyping system as an epidemiological tool.
本文描述了一种基于淋病奈瑟菌在11种化学成分明确的琼脂培养基上的生长情况或生长缺失来区分临床分离株的系统。完全培养基NEDA包含淋球菌生长所需的所有化合物；但分离株在从NEDA中逐个省略特定化合物后生长能力有所不同。这些差异化合物包括L-脯氨酸、L-精氨酸、L-鸟氨酸、L-甲硫氨酸、次黄嘌呤、尿嘧啶、硫胺素和硫胺素焦磷酸。在标准培养基上独特的生长反应模式定义了一个营养缺陷型。在3个月的时间里，从一个城市诊所检查的患者中分离出的251株淋球菌中发现了20种营养缺陷型。从几个不同国家收集的另一组74株菌株又产生了另外两种营养缺陷型。通过两种方式验证了营养缺陷型分型所依赖的营养需求的稳定性。从患者不同解剖部位或性伴侣分离出的培养物代表相同的营养缺陷型，从推定治疗失败病例中反复分离出的培养物也是如此。此外，淋球菌的营养缺陷型在多次传代培养后保持不变。结果的可重复性以及营养缺陷型的种类和数量表明了营养缺陷型分型系统作为一种流行病学工具的潜在价值。
