Catlin B W, Pace P J
Antimicrob Agents Chemother. 1977 Aug;12(2):147-56. doi: 10.1128/AAC.12.2.147.
A system of auxotyping described in 1973 is based on the differing nutritional requirement patterns of Neisseria gonorrhoeae strains. Our ongoing evaluation of the reliability of auxotyping has involved a study of the constancy of characteristics of gonococci isolated at one time from two or more sites of a given subject. The auxotypes and minimal inhibitory concentration (MIC) of penicillin G were determined for 181 isolates obtained from 84 patients with uncomplicated gonorrhea, for 16 isolates from 8 couples with uncomplicated gonorrhea, and for 21 isolates from 12 other patients, 9 with disseminated gonococcal infection and three consorts. The penicillin MIC served to distinguish between many members of auxotypes 1, 2, and 3, which are commonly involved in uncomplicated gonorrhea. Thus, for proline-requiring gonococci (auxotype 2) the MIC ranged from 0.01 to 1.2 IU of penicillin per ml. The profile of gonococcal responses to seven other antibacterial drugs provided useful additional information where the extent of phenotypic similarity was in doubt. In all but seven instances, the gonococci isolated from different sites of the same patient, or from a consort, had the same nutritional requirements and penicillin MIC. The gonococci isolated from one patient with disseminated gonococcal infection and from one of her two sexual contacts had nutritional requirements for arginine, hypoxanthine, uracil, and thiamine pyrophosphate, whereas the strain isolated from her second contact differed in having no requirement for thiamine pyrophosphate. The paired cervical and rectal isolates from one patient with uncomplicated gonorrhea differed only with respect to a requirement for hypoxanthine. Pairs of isolates from three patients differed slightly in degree of susceptibility to penicillin. In the remaining two instances, however, numerous differences between the isolates from the endocervix and the anal canal of a given patient indicated the presence of concomitant infections with different strains of N. gonorrhoeae.
1973年描述的一种辅助分型系统是基于淋病奈瑟菌菌株不同的营养需求模式。我们对辅助分型可靠性的持续评估涉及对从给定受试者的两个或更多部位同时分离出的淋球菌特征稳定性的研究。测定了从84例无并发症淋病患者中分离出的181株菌株、从8对无并发症淋病夫妇中分离出的16株菌株以及从其他12例患者（9例播散性淋球菌感染患者和3名性伴）中分离出的21株菌株的辅助型和青霉素G的最低抑菌浓度（MIC）。青霉素MIC有助于区分通常与无并发症淋病相关的辅助型1、2和3的许多成员。因此，对于需要脯氨酸的淋球菌（辅助型2），MIC范围为每毫升0.01至1.2国际单位青霉素。当表型相似程度存疑时，淋球菌对其他七种抗菌药物的反应情况提供了有用的额外信息。除了七例情况外，从同一患者的不同部位或从性伴分离出的淋球菌具有相同的营养需求和青霉素MIC。从一名播散性淋球菌感染患者及其两名性伴之一分离出的淋球菌对精氨酸、次黄嘌呤、尿嘧啶和硫胺素焦磷酸有营养需求，而从她的另一名性伴分离出的菌株则不需要硫胺素焦磷酸。一名无并发症淋病患者的宫颈和直肠配对分离株仅在对次黄嘌呤的需求方面有所不同。来自三名患者的配对分离株对青霉素的敏感性略有差异。然而，在其余两例中，给定患者宫颈内膜和肛管分离株之间存在许多差异，表明存在不同菌株的淋病奈瑟菌合并感染。
