Muldoon S M, Tyce G M, Moyer T P, Rorie D K
Am J Physiol. 1979 Feb;236(2):H263-7. doi: 10.1152/ajpheart.1979.236.2.H263.
A method for the measurement of norepinephrine (NE) overflow from isolated superfused canine saphenous veins is described. This method involves concentrating NE present in the superfusate, followed by measurement of its concentration by high-performance liquid chromatography with electrochemical detection. The limit of sensitivity of the method (defined as a signal-to-noise ratio of 5) was 25 pg. NE concentration measured by this method correlated well (r = 0.95) with that measured by standard fluorometric methods. Electrical stimulation caused an initial overflow of a mean of 115 X 10(-18) mol NE/mg of vein per pulse; this was associated with an increase in isometric tension. With continued stimulation, less NE overflowed into the superfusate, although tension was not reduced appreciably.
描述了一种测量从离体灌注的犬隐静脉中去甲肾上腺素（NE）溢出量的方法。该方法包括浓缩灌注液中存在的NE，然后通过高效液相色谱-电化学检测法测量其浓度。该方法的灵敏度极限（定义为信噪比为5）为25皮克。用该方法测得的NE浓度与用标准荧光法测得的浓度相关性良好（r = 0.95）。电刺激引起每脉冲平均有115×10⁻¹⁸摩尔NE/毫克静脉的初始溢出；这与等长张力增加有关。随着持续刺激，尽管张力没有明显降低，但溢出到灌注液中的NE减少。
