Devine J E, McDaniel R C
Ann Clin Lab Sci. 1979 Jan-Feb;9(1):80-3.
The brain isoenzyme of creatine kinase (CK) (EC 2.7.3.2) has been identified in the serum of 21 of 950 patients tested. Samples were received over a period of about 20 months. Analysis was performed by electrophoresis on agarose gel with isoenzyme bands visualized by fluorescence after enzyme coupled reactions producing NADH. Specialized substrate preparations were used to rule out the possibility that observed fluorescence was due to enzymatic activity other than that of CK isoenzymes. Patients who were hypothermic and/or had undergone cardiopulmonary resuscitation accounted for 19 of the 21 patients showing the brain isoenzyme. The inciting mechanism for the release of CK1, into the circulation is not clearly understood.
在接受检测的950名患者中，有21名患者的血清中检测到了肌酸激酶（CK）（EC 2.7.3.2）的脑同工酶。样本采集时间约为20个月。通过琼脂糖凝胶电泳进行分析，酶偶联反应产生NADH后，通过荧光观察同工酶条带。使用专门的底物制剂排除观察到的荧光是由CK同工酶以外的酶活性引起的可能性。体温过低和/或接受过心肺复苏的患者占显示脑同工酶的21名患者中的19名。CK1释放到循环中的激发机制尚不清楚。
