Arwert F, Venema G
J Virol. 1974 Mar;13(3):584-9. doi: 10.1128/JVI.13.3.584-589.1974.
The host bacterium of bacteriophage GA-1, Bacillus sp. G1R, was compared with respect to its taxonomic relationship to Bacillus subtilis, B. licheniformis, and B. pumilis. The physiological-biochemical properties of Bacillus sp. G1R are equal to those of B. licheniformis, but the thermal denaturation midpoint of G1R DNA differs by 3 C and the buoyant density by 0.005 g/cm(3) from that of B. licheniformis. Transformation with G1R donor DNA was neither observed in B. licheniformis nor in B. subtilis-competent recipients. Bacteriophage GA-1 shows neither infectivity on B. licheniformis nor on B. subtilis. However, infection of competent B. subtilis cultures with phenol-extracted GA-1 DNA results in the production of infective GA-1 particles. The transfecting activity of GA-1 DNA is destroyed by treatment with proteolytic enzymes. Resistance of transfecting DNA to inactivation by trypsin develops earlier than that to inactivation by DNase. Protease-treated GA-1 DNA competes with transforming DNA to approximately the same extent as does untreated GA-1 DNA, suggesting that uptake of GA-1 DNA is not affected by protease treatment. CsCl density gradient centrifugation reveals that the density of trypsinized GA-1 DNA is 0.004 g/cm(3) greater than that of untreated DNA.
噬菌体GA - 1的宿主细菌芽孢杆菌属G1R，就其与枯草芽孢杆菌、地衣芽孢杆菌和短小芽孢杆菌的分类关系进行了比较。芽孢杆菌属G1R的生理生化特性与地衣芽孢杆菌相同，但G1R DNA的热变性中点与地衣芽孢杆菌相差3℃，浮力密度相差0.005 g/cm³。在地衣芽孢杆菌和枯草芽孢杆菌感受态受体中均未观察到用G1R供体DNA进行的转化。噬菌体GA - 1对地衣芽孢杆菌和枯草芽孢杆菌均无感染性。然而，用酚提取的GA - 1 DNA感染枯草芽孢杆菌感受态培养物会导致产生有感染性的GA - 1颗粒。GA - 1 DNA的转染活性经蛋白水解酶处理后被破坏。转染DNA对胰蛋白酶失活的抗性比对DNA酶失活的抗性出现得更早。经蛋白酶处理的GA - 1 DNA与转化DNA的竞争程度与未处理的GA - 1 DNA大致相同，这表明GA - 1 DNA的摄取不受蛋白酶处理的影响。氯化铯密度梯度离心显示，经胰蛋白酶处理的GA - 1 DNA的密度比未处理的DNA高0.004 g/cm³。
