枯草芽孢杆菌中PBS2的噬菌体转化
Bacteriophage transformation of PBS2 in Bacillus subtilis.
作者信息
Herrington M B, Takahashi I
出版信息
J Virol. 1975 Jan;15(1):16-21. doi: 10.1128/JVI.15.1.16-21.1975.
Abstract
Transformation of temperature-sensitive mutants of bacteriophage PBS2 for Bacillus subtilis was demonstrated. The number of transformants was linearly related to the concentration of DNA within a range of 0.01 to 1 mug/ml. No transformants were obtained when the DNA was pretreated with DNase. PBS2 DNA sheared to approximately 1% of the total chromosome length was centrifuged in Cs2SO4-Hg gradients to fractionate the DNA according to the base composition. Transformation experiments carried out with the fractionated DNA indicated the possibility of determining the base composition of different regions of the phage chromosome.
摘要
已证实噬菌体PBS2的温度敏感突变体可转化枯草芽孢杆菌。在0.01至1微克/毫升的范围内，转化子数量与DNA浓度呈线性关系。用DNA酶预处理DNA后未获得转化子。将剪切至约为总染色体长度1%的PBS2 DNA在Cs2SO4-Hg梯度中离心，以根据碱基组成对DNA进行分级分离。用分级分离后的DNA进行的转化实验表明，有可能确定噬菌体染色体不同区域的碱基组成。
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