关于荧光团长生草烯及其与DNA复合物的研究。
Studies on the fluorophore sempervirene and its complexes with DNA.
作者信息
Burns V W
出版信息
Biophys J. 1974 Mar;14(3):189-99. doi: 10.1016/s0006-3495(74)85906-0.
Abstract
Evidence that the fluorophore sempervirene binds to nucleic acids is presented. The complexes were studied by fluorescence intensity, spectra, decay lifetime, and polarization methods. Both fluorescent and nonfluorescent complexes are formed. The sempervirene is rigidly fixed to DNA. If ethidium bromide and sempervirene are bound to DNA, energy can be transferred from sempervirene to ethidium. Sempervirene is taken up by mammalian cells and appears in the cytoplasm. This unusual new probe should be useful in molecular and cellular investigations.
摘要
本文提供了荧光团万年青烯与核酸结合的证据。通过荧光强度、光谱、衰减寿命和偏振方法对复合物进行了研究。形成了荧光和非荧光复合物。万年青烯被牢固地固定在DNA上。如果溴化乙锭和万年青烯与DNA结合，能量可以从万年青烯转移到溴化乙锭。万年青烯被哺乳动物细胞摄取并出现在细胞质中。这种不同寻常的新探针在分子和细胞研究中应该会很有用。
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