溴化乙锭和双（甲溴化）精胺通过嵌入作用与Z-DNA结合。
Binding of ethidium and bis(methidium)spermine to Z DNA by intercalation.
作者信息
Shafer R H, Brown S C, Delbarre A, Wade D
出版信息
Nucleic Acids Res. 1984 Jun 11;12(11):4679-90. doi: 10.1093/nar/12.11.4679.
Abstract
The interaction of ethidium bromide, a DNA intercalating drug, and bis( methidium )spermine, a DNA bis-intercalating compound, with the left-handed Z form of poly(dG-dC) has been studied in 4.4 M NaCl. Spectrophotometric analysis using absorption, fluorescence and circular dichroism indicates that the complex formed between ethidium and Z DNA resembles very closely that formed with B DNA. This suggests that ethidium binds to Z DNA by intercalation. 31P NMR spectra are presented showing both the conversion of the Z form to the B form with increasing amounts of drug and the typical Z form spectrum at low binding densities. Data are also presented which show that the bifunctional intercalator bis( methidium )spermine binds to Z DNA in a manner similar to its binding to B DNA, i.e., by bis-intercalation. These results are important for our understanding the behavior of Z DNA and its biological significance.
摘要
在4.4M氯化钠溶液中，研究了DNA嵌入药物溴化乙锭和DNA双嵌入化合物双（甲锭）精胺与左手Z型聚（dG-dC）的相互作用。使用吸收、荧光和圆二色性的分光光度分析表明，溴化乙锭与Z-DNA形成的复合物与与B-DNA形成的复合物非常相似。这表明溴化乙锭通过嵌入作用与Z-DNA结合。给出了31P NMR光谱，显示随着药物量的增加，Z型向B型的转变以及低结合密度下典型的Z型光谱。还给出了数据，表明双功能嵌入剂双（甲锭）精胺以类似于其与B-DNA结合的方式与Z-DNA结合，即通过双嵌入作用。这些结果对于我们理解Z-DNA的行为及其生物学意义很重要。
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