一种制备纯UDP[14C]葡萄糖的简化方法。
A simplified method for the preparation of pure UDP[14C] glucose.
作者信息
Tan A W
出版信息
Biochim Biophys Acta. 1979 Feb 1;582(3):543-7. doi: 10.1016/0304-4165(79)90146-6.
PMID:420866
Abstract
A two-step enzymatic synthesis of UDP[14C] glucose was described which resulted in high yield. Separation of product from labelled intermediates and other contaminants was achieved by a simple ion exchange chromatography method.
摘要
描述了一种两步酶促合成UDP[14C]葡萄糖的方法，该方法具有高产率。通过简单的离子交换色谱法实现了产物与标记中间体及其他污染物的分离。
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