链霉菌属中葡萄糖向UDP-4-酮-6-脱氧葡萄糖的酶促转化
Enzymatic conversion of glucose to UDP-4-keto-6-deoxyglucose in Streptomyces spp.
作者信息
Liu S Y, Rosazza J P
机构信息
Division of Medicinal and Natural Products Chemistry and Center for Biocatalysis and Bioprocessing, College of Pharmacy, The University of Iowa, Iowa City, Iowa 52242, USA.
出版信息
Appl Environ Microbiol. 1998 Oct;64(10):3972-6. doi: 10.1128/AEM.64.10.3972-3976.1998.
All of the 2,6-dideoxy sugars contained within the structure of chromomycin A3 are derived from D-glucose. Enzyme assays were used to confirm the presence of hexokinase, phosphoglucomutase, UDPG pyrophosphorylase (UDPGP), and UDPG oxidoreductase (UDPGO), all of which are involved in the pathway of glucose activation and conversion into 2,6-dideoxyhexoses during chromomycin biosynthesis. Levels of the four enzymes in Streptomyces spp. cell extracts were correlated with the production of chromomycins. The pathway of sugar activation in Streptomyces spp. involves glucose 6-phosphorylation by hexokinase, isomerization to G-1-P catalyzed by phosphoglucomutase, synthesis of UDPG catalyzed by UDPGP, and formation of UDP-4-keto-6-deoxyglucose by UDPGO.
嗜铬菌素A3结构中所含的所有2,6-二脱氧糖均源自D-葡萄糖。通过酶分析来确认己糖激酶、磷酸葡萄糖变位酶、尿苷二磷酸葡萄糖焦磷酸化酶（UDPGP）和尿苷二磷酸葡萄糖氧化还原酶（UDPGO）的存在，所有这些酶都参与了嗜铬菌素生物合成过程中葡萄糖的激活途径以及向2,6-二脱氧己糖的转化。链霉菌属细胞提取物中这四种酶的水平与嗜铬菌素的产生相关。链霉菌属中糖的激活途径包括己糖激酶催化葡萄糖6-磷酸化，磷酸葡萄糖变位酶催化异构化为G-1-P，UDPGP催化合成UDPG，以及UDPGO形成UDP-4-酮-6-脱氧葡萄糖。
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