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枯草芽孢杆菌中的无胸腺嘧啶死亡:细胞裂解与脱氧核糖核酸降解之间的相关性。

Thymineless death in Bacillus subtilis: correlation between cell lysis and deoxyribonucleic acid breakdown.

作者信息

Ephrati-Elizur E, Yosuv D, Shmueli E, Horowitz A

出版信息

J Bacteriol. 1974 Jul;119(1):36-43. doi: 10.1128/jb.119.1.36-43.1974.

Abstract

Bacillus subtilis carrying an inducible defective phage is several times more sensitive to thymineless death than a mutagenized derivative that behaves as a nonlysogen. When the integrity of the deoxyribonucleic acid (DNA) of both strains was examined during thymine starvation by transformation experiments, sedimentation studies, and measurements of acid-soluble DNA degradation products, it was shown that extensive DNA breakdown occurred only in the lysogenic strain. During thymine starvation of this strain, there is a progressive proclivity to lysis, followed by leakage of DNA and DNA degradation products. Such leakage was not observed in the nonlysogen. A correlation between proclivity to lysis and extensive DNA degradation is indicated.

摘要

携带诱导型缺陷噬菌体的枯草芽孢杆菌对无胸腺死亡的敏感性比表现为非溶原菌的诱变衍生物高几倍。当通过转化实验、沉降研究和酸溶性DNA降解产物的测量在胸腺嘧啶饥饿期间检查这两种菌株的脱氧核糖核酸(DNA)完整性时,发现只有溶原菌菌株发生了广泛的DNA分解。在该菌株的胸腺嘧啶饥饿期间,存在逐渐裂解的倾向,随后是DNA和DNA降解产物的泄漏。在非溶原菌中未观察到这种泄漏。这表明裂解倾向与广泛的DNA降解之间存在相关性。

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