Lemcke R M, Bew J, Burrows M R, Lysons R J
Res Vet Sci. 1979 May;26(3):315-9.
A new simple method for the preparation of a liquid medium containing rabbit serum for the propagation of Treponema hyodysenteriae and other porcine intestinal spirochaetes is described. The medium, when dispensed in shallow layers and sealed under 10 per cent CO2 in nitrogen, had a redox potential not greater than -125mV and an initial pH of about 6.9 when buffered with bicarbonate. Growth of T hyodysenteriae developed more rapidly and viable counts reached higher levels at 42 degrees C than at 37 degrees C. Viable counts increased at least 10,000-fold after two to five days' incubation, depending on the temperature. Growth could be initiated from small inocula that failed to produce colonies on blood agar. Using a 1 per cent inoculum, the medium supported the growth of two strains of T hyodysenteriae through 10 serial passages.
本文描述了一种制备含有兔血清的液体培养基的新简易方法，用于培养猪痢疾密螺旋体和其他猪肠道螺旋体。该培养基浅层分装并在含10%二氧化碳的氮气中密封时，其氧化还原电位不高于-125mV，用碳酸氢盐缓冲时初始pH约为6.9。猪痢疾密螺旋体在42℃时生长比在37℃时更快，活菌数达到更高水平。根据温度不同，孵育两到五天后活菌数至少增加10000倍。生长可从小接种量开始，这些接种量在血琼脂上不能产生菌落。使用1%的接种量，该培养基支持两株猪痢疾密螺旋体传代10次。
