Miranda A F, Godman G C, Tanenbaum S W
J Cell Biol. 1974 Aug;62(2):406-23. doi: 10.1083/jcb.62.2.406.
Cells in culture exposed to cytochalasin D (CD) rapidly undergo a long-sustained tonic contraction. Coincident with this contracture the thin microfilaments of the cortex become compacted into feltlike masses. The ravelled filaments of these masses remain actinlike and bind heavy meromyosin; they are not disrupted or disaggregated, but rather, appear to represent a contracted state of the microfilament apparatus of the cell cortex. On continued exposure to CD, 'myoid' bundles, containing thick, dense filaments, and larger fusiform or ribbonlike, putatively myosinoid, aggregates may appear. These appearances are interpreted as consequences of a state of hypercontraction without relaxation induced by CD. They do not occur in CD-treated cells prevented from contracting by inhibitors of energy metabolism, and are readily reversible on withdrawal of CD. Extensive ordered arrays of thin microfilaments develop in cells which are reextending during early recovery.
培养的细胞暴露于细胞松弛素D（CD）后会迅速经历长时间持续的强直性收缩。与此挛缩同时发生的是，皮质的细微丝会被压实成毡状团块。这些团块中缠结的细丝仍类似肌动蛋白，并能结合重酶解肌球蛋白；它们并未被破坏或解聚，而是似乎代表了细胞皮质微丝装置的收缩状态。持续暴露于CD时，可能会出现包含粗而致密细丝的“肌样”束，以及更大的梭形或带状、推测为类肌球蛋白的聚集体。这些现象被解释为CD诱导的无松弛的过度收缩状态的结果。它们不会出现在因能量代谢抑制剂而无法收缩的经CD处理的细胞中，并且在撤去CD后很容易逆转。在早期恢复过程中重新伸展的细胞中会形成广泛有序排列的细微丝。
