• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

兔β-葡萄糖醛酸酶。亚细胞分布及免疫化学特性。

Rabbit beta-glucuronidase. Subcellular distribution and immunochemical properties.

作者信息

Dean R T

出版信息

Biochem J. 1974 Mar;138(3):407-13. doi: 10.1042/bj1380407.

DOI:10.1042/bj1380407
PMID:4215419
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1166226/
Abstract
  1. The subcellular distribution of beta-glucuronidase and other hydrolases in rabbit liver was investigated. beta-Glucuronidase was found in both microsomal and lysosomal fractions. 2. Multiple forms of beta-glucuronidase were present in extracts of microsomal and lysosomal fractions. All forms were common to both fractions. 3. A specific antiserum against beta-glucuronidase was raised, and characterized by immunoprecipitation and affinity-chromatography procedures. 4. The immunological identity of the multiple forms in the pure beta-glucuronidase preparation, and the immunological identity of the beta-glucuronidase complement of lysosomal extracts with that of microsomal extracts, were demonstrated by means of the antiserum. The presence of inactive enzyme in various enzyme preparations was shown.
摘要
  1. 对兔肝脏中β-葡萄糖醛酸酶及其他水解酶的亚细胞分布进行了研究。在微粒体和溶酶体组分中均发现了β-葡萄糖醛酸酶。2. 微粒体和溶酶体组分提取物中存在多种形式的β-葡萄糖醛酸酶。所有形式在两个组分中都很常见。3. 制备了针对β-葡萄糖醛酸酶的特异性抗血清，并通过免疫沉淀和亲和层析程序对其进行了表征。4. 借助该抗血清证明了纯β-葡萄糖醛酸酶制剂中多种形式的免疫学同一性，以及溶酶体提取物与微粒体提取物中β-葡萄糖醛酸酶互补物的免疫学同一性。还显示了各种酶制剂中存在无活性酶。
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b308/1166226/a62308c1ee6e/biochemj00586-0108-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b308/1166226/a62308c1ee6e/biochemj00586-0108-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b308/1166226/a62308c1ee6e/biochemj00586-0108-a.jpg

相似文献

1
Rabbit beta-glucuronidase. Subcellular distribution and immunochemical properties.兔β-葡萄糖醛酸酶。亚细胞分布及免疫化学特性。
Biochem J. 1974 Mar;138(3):407-13. doi: 10.1042/bj1380407.
2
Purification and characterization of microsomal and lysosomal beta-glucuronidase from rat liver by use of immunoaffinity chromatography.利用免疫亲和层析法对大鼠肝脏微粒体和溶酶体β-葡萄糖醛酸酶进行纯化及特性分析。
Eur J Biochem. 1976 Nov 15;70(2):349-59. doi: 10.1111/j.1432-1033.1976.tb11024.x.
3
Studies on cathepsins of rat liver lysosomes. I. Purification and multiple forms.
J Biochem. 1974 Sep;76(3):639-49. doi: 10.1093/oxfordjournals.jbchem.a130608.
4
Intracellular distribution of steroid glycosidases of rabbit liver.兔肝脏甾体糖苷酶的细胞内分布
Can J Biochem. 1973 Sep;51(9):1292-6. doi: 10.1139/o73-169.
5
Multiple forms of beta-glucuronidase in rat liver lysosomes and microsomes.大鼠肝脏溶酶体和微粒体中β-葡萄糖醛酸酶的多种形式。
Arch Biochem Biophys. 1975 Jan;166(1):258-72. doi: 10.1016/0003-9861(75)90387-2.
6
Purification and characterization of rat liver microsomal beta-glucuronidase.大鼠肝脏微粒体β-葡萄糖醛酸酶的纯化与特性分析
Biochim Biophys Acta. 1976 Jul 8;438(2):474-86. doi: 10.1016/0005-2744(76)90263-1.
7
Isolation of rat liver lysosomes by loading with colloidal gold.通过加载胶体金分离大鼠肝脏溶酶体。
Biochim Biophys Acta. 1974 Jun 20;354(1):114-20. doi: 10.1016/0304-4165(74)90059-2.
8
Heterogeneity of Wistar rats with respect to acid beta-D-glucuronidase (EC 3.2.1.31) in liver.
Biochem Mol Biol Int. 1994 May;33(2):235-44.
9
The subcellular distribution of lysosomal hydrolases in the Harding-Passey melanoma.哈丁-帕西黑色素瘤中溶酶体水解酶的亚细胞分布
J Cell Physiol. 1974 Feb;83(1):75-83. doi: 10.1002/jcp.1040830111.
10
A new method for the preparation of rat liver lysosomes. Separation of cell organelles of rat liver by carrier-free continuous electrophoresis.一种制备大鼠肝脏溶酶体的新方法。通过无载体连续电泳分离大鼠肝脏的细胞器。
J Cell Biol. 1970 Sep;46(3):576-91. doi: 10.1083/jcb.46.3.576.

引用本文的文献

1
A survey on the formation and localization of secondary isozymes in mammalia.哺乳动物中次生同工酶的形成与定位研究
Hum Genet. 1980;56(2):129-55. doi: 10.1007/BF00295687.
2
Rabbit beta-glucuronidase. Purification and properties, and the existence of multiple forms.兔β-葡萄糖醛酸酶。纯化与特性，以及多种形式的存在。
Biochem J. 1974 Mar;138(3):395-405. doi: 10.1042/bj1380395.
3
Biogenesis of microsomal membrane glycoproteins in rat liver. III. Release of glycoproteins from the Golgi fraction and their transfer to microsomal membranes.

本文引用的文献

1
The effect of mutation on the intracellular location of beta-glucuronidase.突变对β-葡萄糖醛酸酶细胞内定位的影响。
Exp Cell Res. 1961 Nov;25:286-301. doi: 10.1016/0014-4827(61)90280-4.
2
Tissue fractionation studies. 6. Intracellular distribution patterns of enzymes in rat-liver tissue.组织分级分离研究。6. 大鼠肝脏组织中酶的细胞内分布模式。
Biochem J. 1955 Aug;60(4):604-17. doi: 10.1042/bj0600604.
3
Comparison of the effects of various detergents on antigen-antibody interaction.各种去污剂对抗抗原-抗体相互作用的影响比较。
大鼠肝脏微粒体膜糖蛋白的生物合成。III. 糖蛋白从高尔基体部分的释放及其向微粒体膜的转移。
J Cell Biol. 1975 Dec;67(3):715-24. doi: 10.1083/jcb.67.3.715.
4
Human beta-glucuronidase: assignment of the structural gene to chromosome 7 using somatic cell hybrids.人β-葡萄糖醛酸酶：利用体细胞杂种将结构基因定位于7号染色体
Biochem Genet. 1977 Apr;15(3-4):367-82. doi: 10.1007/BF00484467.
5
Application of enzymehistochemical methods to isolated subcellular fractions and to sucrose-ficoll density gradients. A contribution to the comparison of histochemical and biochemical data.酶组织化学方法在分离的亚细胞组分和蔗糖-菲可密度梯度中的应用。对组织化学和生物化学数据比较的一项贡献。
Histochemistry. 1977 Aug 1;53(2):165-81. doi: 10.1007/BF00498491.
6
Immunochemical studies with a specific antiserum to rabbit fibroblast collagenase.用针对兔成纤维细胞胶原酶的特异性抗血清进行的免疫化学研究。
Biochem J. 1975 Dec;151(3):655-63. doi: 10.1042/bj1510655.
7
Absorption of antisera for studies on specific enzyme turnover.用于特定酶周转研究的抗血清的吸收
Biochem J. 1976 Nov;159(2):355-62. doi: 10.1042/bj1590355.
FEBS Lett. 1972 May 1;22(2):210-212. doi: 10.1016/0014-5793(72)80047-4.
4
Separation and properties of beta-galactosidase, beta-glucosidase, beta-glucuronidase and N-acetyl-beta-glucosaminidase from rat kidney.大鼠肾脏中β-半乳糖苷酶、β-葡萄糖苷酶、β-葡萄糖醛酸酶和N-乙酰-β-葡萄糖胺酶的分离及性质
Biochem J. 1967 Feb;102(2):525-32. doi: 10.1042/bj1020525.
5
Inhibition of beta-glucuronidase by cholesterol and retinol.胆固醇和视黄醇对β-葡萄糖醛酸酶的抑制作用。
J Biol Chem. 1967 May 25;242(10):2463-9.
6
p-(acetoxymercuric) aniline diazotate, a reagent for visualizing the naphthol AS-BI product of acid hydrolase action at the level of the light and electron microscope.对（乙酰氧基汞）苯胺重氮酸盐，一种用于在光学显微镜和电子显微镜水平上可视化酸性水解酶作用产生的萘酚AS-BI产物的试剂。
J Histochem Cytochem. 1969 Jan;17(1):1-22. doi: 10.1177/17.1.1.
7
Immunochemical studies on beta-glucuronidase isoenzyme.
Med J Osaka Univ. 1968 Dec;19(2):147-55.
8
The cellular distribution of some rat-kidney glycosidases.一些大鼠肾脏糖苷酶的细胞分布。
Biochem J. 1967 Nov;105(2):877-83. doi: 10.1042/bj1050877.
9
Chemical fixation of enzymes to cyanogen halide activated polysaccharide carriers.酶与卤化氰活化多糖载体的化学固定
Eur J Biochem. 1971 Feb 1;18(3):351-60. doi: 10.1111/j.1432-1033.1971.tb01250.x.
10
Study on the site of biosynthesis of beta-glucuronidase and its appearance in lysosome in normal and hypoxic rats.正常和低氧大鼠中β-葡萄糖醛酸酶的生物合成位点及其在溶酶体中的出现研究。
J Histochem Cytochem. 1970 Aug;18(8):529-41. doi: 10.1177/18.8.529.