噬菌体λ与真核生物DNA的活性分子杂交体。

Thomas M, Cameron J R, Davis R W

Proc Natl Acad Sci U S A. 1974 Nov;71(11):4579-83. doi: 10.1073/pnas.71.11.4579.

A bacteriophage lambda strain has been constructed and a method developed by which DNA from potentially any source can be covalently inserted through EcoRI cohesive ends into the middle of the lambda DNA. These hybrid DNAs can infect nonrestricting Escherichia coli cells and can then propagate as plaque-forming phage. A unique feature of this lambda strain is that extra DNA in the middle of its genome is required for plaque formation. A large number of such phages have been produced with E. coli DNA and Drosophila melanogaster DNA.

构建了一种λ噬菌体菌株，并开发了一种方法，通过该方法，来自任何潜在来源的DNA都可以通过EcoRI粘性末端共价插入到λDNA的中间。这些杂交DNA可以感染非限制性大肠杆菌细胞，然后作为形成噬菌斑的噬菌体进行繁殖。这种λ噬菌体菌株的一个独特特征是，其基因组中间的额外DNA是形成噬菌斑所必需的。已经用大肠杆菌DNA和黑腹果蝇DNA产生了大量这样的噬菌体。