非痰液标本的分枝杆菌培养
Culture of specimens other than sputum for Mycobacteria.
作者信息
Mitchison A, Aber V R
出版信息
J Clin Pathol. 1974 Nov;27(11):883-7. doi: 10.1136/jcp.27.11.883.
Abstract
A comparison has been made of Lowenstein-Jensen medium, with or without added pyruvate, 7H11 oleic acid-albumin agar slopes made selective by the addition of antibiotics, and selective Kirchner medium in the isolation of tubercle bacilli from specimens other than sputum. A combination of a standard mild preliminary treatment with sulphuric acid and selective medium reduced the contamination rate to 0.1%. Neither acid treatment nor antibiotics appeared to reduce the isolation rate. An effective procedure is to treat all specimens (except cerebrospinal fluid) with acid and culture on two Lowenstein-Jensen slopes (one with pyruvate), a 7H11 selective slope, and in two bottles of selective Kirchner medium.
摘要
对罗-琴培养基（添加或不添加丙酮酸盐）、添加抗生素制成选择性培养基的7H11油酸-白蛋白琼脂斜面以及用于从痰液以外标本中分离结核杆菌的选择性柯赫纳培养基进行了比较。标准的温和硫酸预处理与选择性培养基相结合可将污染率降至0.1%。酸处理和抗生素似乎均未降低分离率。一种有效的方法是用酸处理所有标本（脑脊液除外），并接种在两个罗-琴斜面上（一个添加丙酮酸盐）、一个7H11选择性斜面以及两瓶选择性柯赫纳培养基中进行培养。
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