一种用于培养牛分枝杆菌的选择性油酸白蛋白琼脂培养基。
A selective oleic acid albumin agar medium for the cultivation of Mycobacterium bovis.
作者信息
Gallagher J, Horwill D M
出版信息
J Hyg (Lond). 1977 Aug;79(1):155-60. doi: 10.1017/s0022172400052943.
Abstract
The modification of Middlebrook's 7H11 oleic acid albumin agar medium by the addition of fresh bovine serum and lysed sheep red cells to encourage growth of Mycobacterium bovis is described. The improved medium was made selective by the addition of antibiotics and a comparative trial of this medium and the guineapig test in the isolation of M. bovis from badger tissues is reported. A close agreement between the two tests was found; the guinea-pig test detected 95% of all isolations and culture detected 91%.
摘要
描述了通过添加新鲜牛血清和裂解的绵羊红细胞来改良Middlebrook氏7H11油酸白蛋白琼脂培养基以促进牛分枝杆菌生长的方法。通过添加抗生素使改良后的培养基具有选择性，并报告了该培养基与豚鼠试验在从獾组织中分离牛分枝杆菌方面的比较试验。发现两种试验结果高度一致；豚鼠试验检测出所有分离物的95%，培养检测出91%。
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