豚鼠外周气道体外过敏反应

Anaphylaxis in guinea-pig peripheral airways in vitro.

作者信息

Mitchell H W, Denborough M A

出版信息

Eur J Pharmacol. 1979 Feb 15;54(1-2):69-78. doi: 10.1016/0014-2999(79)90409-6.

Abstract

The contributions made by histamine, prostaglandins (PG) and slow reacting substance (SRS-A) to anaphylactic contractions in isolated guinea-pig lung were investigated. Histamine, PGF2alpha and SRS-A induced isometric contractions in lung parenchymal strips and in tracheal smooth muscle. The effective concentrations of the autacoids were the same in lung strips and tracheas. Anaphylactic contractions in lung strips from bovine serum albumin sensitised animals were not affected by mepyramine (5 X 10(-6)M), or indomethacin (3 X 10(-5)M) but were markedly reduced by FPL 55712 (5 X 10(-5)M) suggesting that SRS-A plays a major role in the anaphylactic response. The effect of isoprenaline on anaphylactic histamine release and contractions was also examined. Anaphylactic contractions in lung strips were inhibited by isoprenaline (10(-10)--10(-7)M). Isoprenaline also inhibited anaphylactic histamine release from lung parenchyma. 10(-7)M isoprenaline inhibited contractions by 85.6% and reduced histamine release by 27.9%. The results suggest that histamine release does not contribute markedly to anaphylaxis in peripheral airways.

摘要

研究了组胺、前列腺素（PG）和慢反应物质（SRS-A）对豚鼠离体肺过敏性收缩的作用。组胺、前列腺素F2α（PGF2α）和SRS-A可引起肺实质条带和气管平滑肌的等长收缩。这些自体活性物质在肺条带和气管中的有效浓度相同。用牛血清白蛋白致敏动物的肺条带中的过敏性收缩不受甲氧苄胺（5×10⁻⁶M）或消炎痛（3×10⁻⁵M）的影响，但FPL 55712（5×10⁻⁵M）可使其明显减弱，这表明SRS-A在过敏反应中起主要作用。还研究了异丙肾上腺素对过敏性组胺释放和收缩的影响。异丙肾上腺素（10⁻¹⁰ - 10⁻⁷M）可抑制肺条带中的过敏性收缩。异丙肾上腺素还可抑制肺实质中过敏性组胺的释放。10⁻⁷M异丙肾上腺素可使收缩抑制85.6%，组胺释放减少27.9%。结果表明，组胺释放对外周气道过敏反应的贡献不明显。