Dmowski W P, Gaynor L, Lawrence M, Rao R, Scommegna A
Fertil Steril. 1979 Jan;31(1):58-62. doi: 10.1016/s0015-0282(16)43761-1.
Semen samples obtained from 27 infertile men were separated on human serum albumin (HSA) columns prior to artificial homologous insemination (AIH). The columns contained either a single 7.5% HSA layer or two 17.5% and 7.5% HSA layers. Separated specimens were free of seminal debris, had significantly improved motility and progressive drive, and had a decreased percentage of abnormal forms. The total sperm count was also significantly decreased after separation, especially with the two-layer technique. No conceptions occurred in 21 couples when two-layer separation was used, but four pregnancies in 12 couples resulted from AIH with semen separated on the single-layer column. The mean total count of motile sperm obtained for AIH was 5 million with the two-layer and 31 million with the one-layer technique.
从27名不育男性身上获取的精液样本，在进行人工同源授精（AIH）之前，先在人血清白蛋白（HSA）柱上进行分离。这些柱子包含一个单一的7.5% HSA层或两个17.5%和7.5% HSA层。分离后的标本没有精液碎片，活力和向前驱动力显著提高，异常形态的百分比降低。分离后总精子数也显著减少，尤其是采用双层技术时。采用双层分离时，21对夫妇未受孕，但12对夫妇采用单层柱分离精液进行AIH后有4例怀孕。双层技术用于AIH时，活动精子的平均总数为500万，单层技术为3100万。
