Hanson R W, Ziporin Z Z
J Lipid Res. 1966 Jan;7(1):56-61.
Factors influencing the utilization of ketone bodies by mouse adipose tissue in vitro were studied. Epididymal fat pads can oxidize DL-Beta-hydroxybutyrate-3-(14)C and acetoacetate-3-(14)C to (14)CO(2) as well as convert these compounds to fatty acid-(14)C. An increased output of (14)CO(2) from Beta-hydroxybutyrate-3-(14)C was noted in response to glucose plus insulin, succinate, oxaloacetate, L-asparate, and L-malate. Fatty acid synthesis from Beta-hydroxybutyrate was enhanced by glucose plus insulin, L-aspartate, L-malate, oxaloacetate, and citrate. Nicotinamide stimulated the oxidation of Beta-hydroxybutyrate but not of acetoacetate to CO(2), and did not affect fatty acid synthesis from either ketone body. Nicotinamide increased NAD(+) and NADP(+) levels in epididymal fat pads without affecting the concentration of NADH and NADPH. "Superlipogenesis" caused by fasting the mice for 48 hr and re-feeding them for 24 hr sharply enhanced CO(2) output and lipogenesis from Beta-hydroxybutyrate. The activities of glucose-6-phosphate dehydrogenase, 6-phosphogluconic dehydrogenase, NADP-malic dehydrogenase, and citrate cleavage enzyme from mouse adipose tissue were increased during "superlipogenesis." Free fatty acid release by epididymal fat pads in vitro was slightly increased by Beta-hydroxybutyrate. The relationship of ketone body metabolism and lipogenesis in adipose tissue is discussed.
研究了影响小鼠脂肪组织体外利用酮体的因素。附睾脂肪垫能够将DL-β-羟基丁酸-3-(14)C和乙酰乙酸-3-(14)C氧化为(14)CO(2)，并将这些化合物转化为脂肪酸-(14)C。观察到在葡萄糖加胰岛素、琥珀酸、草酰乙酸、L-天冬氨酸和L-苹果酸的作用下，β-羟基丁酸-3-(14)C产生的(14)CO(2)输出增加。葡萄糖加胰岛素、L-天冬氨酸、L-苹果酸、草酰乙酸和柠檬酸可增强由β-羟基丁酸合成脂肪酸的过程。烟酰胺刺激β-羟基丁酸氧化为CO(2)，但不刺激乙酰乙酸氧化为CO(2)，且不影响由任一酮体合成脂肪酸的过程。烟酰胺可提高附睾脂肪垫中NAD(+)和NADP(+)水平，而不影响NADH和NADPH的浓度。小鼠禁食48小时后再喂食24小时所导致的“超级脂肪生成”显著增强了β-羟基丁酸的CO(2)输出和脂肪生成。在“超级脂肪生成”过程中，小鼠脂肪组织中葡萄糖-6-磷酸脱氢酶、6-磷酸葡萄糖酸脱氢酶、NADP-苹果酸脱氢酶和柠檬酸裂解酶的活性增加。附睾脂肪垫在体外释放的游离脂肪酸可被β-羟基丁酸轻微增加。本文讨论了脂肪组织中酮体代谢与脂肪生成的关系。
