Doner L W, White J W, Phillips J G
J Assoc Off Anal Chem. 1979 Jan;62(1):186-9.
A gas-liquid chromatographic (GLC) method has been developed to detect the addition to honey of high fructose corn sirup (HFCS). Samples are derivatized directly with trimethylsilane, cholestane is added as an internal standard, and the levels of maltose (includes other minor disaccharides) and isomaltose are determined after chromatography on OV-17. Domestic and imported honey samples (115) contained 2.00% maltose and 0.71% isomaltose. HFCS samples (21) contained 1.50% maltose, and 2.09% isomaltose. A discriminatory equation was developed (D = 2.73 - 5. 35 (Isomaltose/maltose)) and, when applied to the data for these samples and 37 adulterated samples, 81.4% of authentic honey samples and 78.4% of samples known to be adulterated with HFCS were correctly classified.
已开发出一种气液色谱（GLC）方法来检测蜂蜜中添加的高果糖玉米糖浆（HFCS）。样品直接用三甲基硅烷衍生化，加入胆甾烷作为内标，在OV - 17上进行色谱分析后测定麦芽糖（包括其他少量二糖）和异麦芽糖的含量。国内和进口蜂蜜样品（115个）含有2.00%的麦芽糖和0.71%的异麦芽糖。HFCS样品（21个）含有1.50%的麦芽糖和2.09%的异麦芽糖。建立了一个判别方程（D = 2.73 - 5.35（异麦芽糖/麦芽糖）），当将其应用于这些样品和37个掺假样品的数据时，81.4%的纯正蜂蜜样品和78.4%已知掺有HFCS的样品被正确分类。
