Kushnir I
J Assoc Off Anal Chem. 1979 Jul;62(4):917-20.
A highly sensitive procedure has been developed to detect the undeclared addition of high fructose corn sirup (HFCS) to honey. Carbohydrates must be separated first to achieve the requisite degree of sensitivity; charcoal-Celite chromatography was used to isolate a fraction containing oligo- and polysaccharides. The fraction was then concentrated and examined by thin layer chromatography on silica gel. Pure honeys yielded only 1 or 2 blue-grey or blue-brown spots at Rf values greater than 0.35; a series of spots or blue streaks extending from the origin characterized adulterated samples. The method detects HFCS and conventional honey adulterants at levels as low as 10% or less of the total mixture. In addition, the procedure detects the presence in honey of all starch-derived sugar sirups tested thus far, regardless of the plant source.
已开发出一种高灵敏度的方法来检测蜂蜜中未申报添加的高果糖玉米糖浆（HFCS）。必须首先分离碳水化合物以达到所需的灵敏度；采用活性炭-硅藻土色谱法分离出含有寡糖和多糖的馏分。然后将该馏分浓缩，并通过硅胶薄层色谱法进行检测。纯蜂蜜在比移值大于0.35时仅产生1或2个蓝灰色或蓝棕色斑点；一系列从原点延伸的斑点或蓝色条纹是掺假样品的特征。该方法能检测出HFCS和传统蜂蜜掺假物，其含量低至总混合物的10%或更低。此外，该方法还能检测出迄今为止测试的所有淀粉衍生糖蜜在蜂蜜中的存在情况，无论其植物来源如何。
