在正常成纤维细胞与转化成纤维细胞之间的异核体中，纤连蛋白的表达由转化亲代细胞的基因型决定。
Fibronectin expression is determined by the genotype of the transformed parental cells in heterokaryons between normal and transformed fibroblasts.
作者信息
Laurila P, Wartiovaara J, Stenman S
出版信息
J Cell Biol. 1979 Jan;80(1):118-27. doi: 10.1083/jcb.80.1.118.
Abstract
The expression of fibronectin, a cell surface-associated transformation-sensitive glycoprotein, was studied in hetero- and homokaryons of normal and SV40-transformed human fibroblasts. In immunofluorescence, fibroblast homokaryons had an intense surface-associated and intracelluar fibronectin fluorescence similar to that of normal fibroblasts. Transformed cells and their homokaryons had a minimal surface-associated and a weak intracellular fibronectin fluorescence. In heterokaryons formed between transformed and normal fibroblasts, the expression of fibronectin fell within 24 h to the level of the transformed cell homokaryons. The change was detectable already at 3 h after fusion and was gene-dose dependent. These results show that the transformed genotype determines fibronectin expression in the heterokaryons.
摘要
纤连蛋白是一种与细胞表面相关的转化敏感糖蛋白，对其在正常和SV40转化的人成纤维细胞的异核体和同核体中的表达进行了研究。在免疫荧光实验中，成纤维细胞同核体具有强烈的表面相关和细胞内纤连蛋白荧光，类似于正常成纤维细胞。转化细胞及其同核体具有最小的表面相关和微弱的细胞内纤连蛋白荧光。在转化和成纤维细胞之间形成的异核体中，纤连蛋白的表达在24小时内降至转化细胞同核体的水平。这种变化在融合后3小时即可检测到，并且是基因剂量依赖性的。这些结果表明，转化的基因型决定了异核体中纤连蛋白的表达。
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